Lightbody, Kirsty L., Renshaw, Philip S., Collins, Michelle L., Wright, Rebecca L., Hunt, Debbie M., Gordon, Stephen V., Hewinson, R. Glyn, Buxton, Roger S., Williamson, Richard A., Carr, Mark D. and others. (2004) Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility. FEMS Microbiol Lett, 238 (1). pp. 255-262. ISSN 0378-1097. (doi:10.1016/j.femsle.2004.07.043) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:5310)
| The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
| Official URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... |
Abstract
We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pairs of CFP-10/ESAT-6 family proteins (Rv0287/Rv0288 and Rv3019c/Rv3020c) and to determine whether complexes can be formed between non-genome paired members of the family. The results clearly demonstrate that Rv0287/Rv0288 and Rv3019c/3020c form tight complexes, as initially observed for CFP-10/ESAT-6. The closely related Rv0287/Rv0288 and Rv3019c/Rv3020c proteins are also able to form non-genome paired complexes (Rv0287/Rv3019c and Rv0288/Rv3020c), but are not capable of binding to the more distantly related CFP-10/ESAT-6 proteins.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.1016/j.femsle.2004.07.043 |
| Additional information: | 0378-1097 (Print) Journal Article Research Support, Non-U.S. Gov't |
| Uncontrolled keywords: | Antigens, Bacterial/genetics/*metabolism Bacterial Proteins/genetics/*metabolism Genome, Bacterial *Multiprotein Complexes Mycobacterium tuberculosis/*metabolism Phylogeny Protein Binding Sequence Homology Two-Hybrid System Techniques beta-Galactosidase/analysis |
| Subjects: | Q Science |
| Divisions: | Divisions > Division of Natural Sciences > Biosciences |
| Depositing User: | Richard Williamson |
| Date Deposited: | 09 Sep 2008 15:04 UTC |
| Last Modified: | 05 Nov 2024 09:37 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/5310 (The current URI for this page, for reference purposes) |
- Export to:
- RefWorks
- EPrints3 XML
- BibTeX
- CSV
- Depositors only (login required):
Altmetric
Altmetric
