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Expansion of the HSFY gene family in pig lineages: HSFY expansion in suids

Skinner, Benjamin M., Lachani, Kim, Sargent, Carole A, Yang, Fengtang, Ellis, Peter J.I., Hunt, Toby, Fu, Beiyuan, Louzada, Sandra, Churcher, Carol, Tyler-Smith, Chris, and others. (2015) Expansion of the HSFY gene family in pig lineages: HSFY expansion in suids. BMC Genomics, 16 (442). pp. 1-11. ISSN 1471-2164. (doi:10.1186/s12864-015-1650-x)

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http://doi.org/10.1186/s12864-015-1650-x

Abstract

BACKGROUND: Amplified gene families on sex chromosomes can harbour genes with important biological functions, especially relating to fertility. The Y-linked heat shock transcription factor (HSFY) family has become amplified on the Y chromosome of the domestic pig (Sus scrofa), in an apparently independent event to an HSFY expansion on the Y chromosome of cattle (Bos taurus). Although the biological functions of HSFY genes are poorly understood, they appear to be involved in gametogenesis in a number of mammalian species, and, in cattle, HSFY gene copy number may correlate with levels of fertility. RESULTS: We have investigated the HSFY family in domestic pig, and other suid species including warthog, bushpig, babirusa and peccaries. The domestic pig contains at least two amplified variants of HSFY, distinguished predominantly by presence or absence of a SINE within the intron. Both these variants are expressed in testis, and both are present in approximately 50 copies each in a single cluster on the short arm of the Y. The longer form has multiple nonsense mutations rendering it likely non-functional, but many of the shorter forms still have coding potential. Other suid species also have these two variants of HSFY, and estimates of copy number suggest the HSFY family may have amplified independently twice during suid evolution. CONCLUSIONS: The HSFY genes have become amplified in multiple species lineages independently. HSFY is predominantly expressed in testis in domestic pig, a pattern conserved with cattle, in which HSFY may play a role in fertility. Further investigation of the potential associations of HSFY with fertility and testis development may be of agricultural interest.

Item Type: Article
DOI/Identification number: 10.1186/s12864-015-1650-x
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
S Agriculture > SF Animal husbandry
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Peter Ellis
Date Deposited: 06 Oct 2015 22:05 UTC
Last Modified: 29 May 2019 16:05 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/50792 (The current URI for this page, for reference purposes)
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