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Flying spot en-face OCT for monitoring cell distribution in collagen-based constructs

Kosmidis, K., Russell, C.D., Black, R.A., Dobre, George, Podoleanu, Adrian G.H. (2006) Flying spot en-face OCT for monitoring cell distribution in collagen-based constructs. In: SPIE Proceedings Series. Proceedings of SPIE - The International Society for Optical Engineering. 6079. Spie-Int Soc Optical Engineering ISBN 0-8194-6121-0. (doi:10.1117/12.648886) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1117/12.648886

Abstract

The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.

Item Type: Conference or workshop item (Proceeding)
DOI/Identification number: 10.1117/12.648886
Uncontrolled keywords: Cell distribution, Collagen gels, Optical Coherence Tomography, Tissue Engineering,
Subjects: Q Science > QC Physics
R Medicine > R Medicine (General) > R857.O6 Optical coherence tomography
Divisions: Faculties > Sciences > School of Physical Sciences > Applied Optics Group
Depositing User: Giles Tarver
Date Deposited: 24 Jul 2015 14:53 UTC
Last Modified: 01 Aug 2019 10:39 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/49753 (The current URI for this page, for reference purposes)
Dobre, George: https://orcid.org/0000-0002-5695-2591
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