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Isolation and characterisation of Mauritius lowland day gecko Phelsuma guimbeaui microsatellite loci

Buckland, Steeves, Horsburgh, Gavin J., Dawson, Deborah A., Cole, Nik C., Krupa, Andrew P., Gallagher, Laura E., Henshaw, Sion M., Groombridge, Jim J., Harris, Stephen (2013) Isolation and characterisation of Mauritius lowland day gecko Phelsuma guimbeaui microsatellite loci. Conservation Genetics Resources, 5 (4). pp. 1013-1018. ISSN 1877-7252. (doi:10.1007/s12686-013-9957-x) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://doi.org/10.1007/s12686-013-9957-x

Abstract

We isolated 315 sequences from a Phelsuma guimbeaui microsatellite-enriched genomic library. Primer sets were designed for 44 loci and used to genotype 29 unrelated individuals belonging to a population in the west of Mauritius. All the loci were polymorphic and the number of alleles ranged from 6 to 34. Mean observed and expected heterozygosity varied from 0.29 to 1.00 and 0.48 to 0.97, respectively. Thirteen loci displayed evidence of deviation from Hardy–Weinberg equilibrium, of which 11 also had an estimated null allele frequency ?10 %. The microsatellite loci will be used to evaluate the population structure and genetic diversity of P. guimbeaui in Mauritius.

Item Type: Article
DOI/Identification number: 10.1007/s12686-013-9957-x
Uncontrolled keywords: Conservation Gekkonidae Microsatellite marker Phelsuma Reptile Simple sequence repeats (SSRs)
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QH Natural history > QH541 Ecology
Q Science > QH Natural history > QH75 Conservation (Biology)
Divisions: Faculties > Social Sciences > School of Anthropology and Conservation > DICE (Durrell Institute of Conservation and Ecology)
Depositing User: Jim Groombridge
Date Deposited: 08 May 2015 14:23 UTC
Last Modified: 29 May 2019 14:31 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/48316 (The current URI for this page, for reference purposes)
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