Alignment of the chicken linkage map with BAC contigs

Romanov, Michael N and Suchyta, S P and Peters, E and Sizemore, F G and Dodgson, Jerry B (2002) Alignment of the chicken linkage map with BAC contigs. In: Final Abstracts Guide of the International Plant, Animal and Microbe Genome X Conference. Scherago International, San Diego, CA, USA 225, Abstract P588. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Abstract

The long term goal of our research is to align the chicken genetic linkage map, defined primarily by segregation of microsatellite and other DNA-based markers, with the anticipated BAC contig physical map of the chicken genome now being generated and eventually with the full genome sequence. Most results to date derive from hybridization to filter-spotted arrays containing about 39,000 BAC clones (one-third of our complete library). As an initial test, PCR-amplified DNA fragments of 31 markers from 10 linkage groups were labeled and hybridized in pools. Positive BAC colonies were confirmed by individual probe hybridization to BAC clone Southern blots and/or PCR amplification with primers derived from the marker sequence, generating 79 confirmed BAC alignments for this collection of probes. To increase throughput, we are now using pooled OVERGO hybridization probes. The primary advantage of OVERGO probes is that the sequence of all members of each pool can be adjusted to have similar melting temperatures. A 6x6x6 matrix of 216 probes based on markers on chromosomes 1 and 2 has been employed. Three-dimensional (18 pools) or four-dimensional (24 pools) hybridization is being used. Results focused on chromosomes 1, 2 and 17 will be presented. Additional screens using a 60,000 BAC subset of the total 115,000 BAC library are planned for other chicken linkage groups.

Item Type: Conference or workshop item (Poster)
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculties > Sciences > School of Biosciences > Biomedical Research Group
Depositing User: M. Romanov
Date Deposited: 15 Jan 2015 23:10 UTC
Last Modified: 27 Aug 2016 16:32 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/46712 (The current URI for this page, for reference purposes)
Romanov, Michael N: https://orcid.org/0000-0003-3584-4644
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