Novel tools for characterising inter- and intra-chromosomal rearrangements in avian microchromosomes

Lithgow, Pamela E and O'Connor, Rebecca and Smith, Deborah and Fonseka, Gothami and Rathje, Claudia and Frodsham, Richard and O’Brien, Patricia C and Ferguson-Smith, Malcolm A and Skinner, Benjamin M and Griffin, Darren K. and Romanov, Michael N (2014) Novel tools for characterising inter- and intra-chromosomal rearrangements in avian microchromosomes. In: 2014 Meeting on Avian Model Systems, 5-8 March 2014, Cold Spring Harbor, NY, USA. (doi:https://doi.org/10.1007/s10577-014-9435-7) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Abstract

Avian genome organization is characterized, in part, by a set of microchromosomes that are unusually small in size and unusually large in number. Although occupying about a quarter of the genome, they contain around half the genes and three quarters of the chromosome count. Nonetheless, they continue to belie analysis by cytogenetic means. Chromosomal rearrangements play a key role in genome evolution, fertility and genetic disease and, thus, tools for the microchromosomes are essential to analyze such phenomena in birds. Here we report the development of chicken microchromosomal paint pools, generation of pairs of specific microchromosome BAC clones in chicken, and tools for in-silico genomic comparison of microchromosomes. We demonstrate the application of these tools cross-species, which can, in principle, be used in combination to generate a clear picture of microchromosomal rearrangements for numerous avian species. With increasing numbers of avian genome sequences being generated, tools such as these will find great utility in assembling genomes de-novo and asking fundamental questions about genome evolution from a chromosome perspective.

Item Type: Conference or workshop item (Poster)
Uncontrolled keywords: evolutionary and comparative cytogenetics; biochemistry & molecular biology; genetics & heredity
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculties > Sciences > School of Biosciences > Biomedical Research Group
Depositing User: M. Romanov
Date Deposited: 14 Jan 2015 16:10 UTC
Last Modified: 21 Aug 2018 08:37 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/46692 (The current URI for this page, for reference purposes)
O'Connor, Rebecca: https://orcid.org/0000-0002-4270-970X
Romanov, Michael N: https://orcid.org/0000-0003-3584-4644
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