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Deficiency in the multicopy Sycp3-like X-linked genes Slx and Slxl1 causes major defects in spermatid differentiation.

Cocquet, Julie, Ellis, Peter J.I., Yamauchi, Yasuhiro, Riel, Jonathan M., Karacs, Thomas P.S., Rattigan, Aine, Ojarikre, Obah A., Affara, Nabeel A., Ward, Monika A., Burgoyne, Paul S and others. (2010) Deficiency in the multicopy Sycp3-like X-linked genes Slx and Slxl1 causes major defects in spermatid differentiation. Molecular biology of the cell, 21 (20). pp. 3497-505. ISSN 1939-4586. (doi:10.1091/mbc.e10-07-0601) (KAR id:46552)


The human and mouse sex chromosomes are enriched in multicopy genes required for postmeiotic differentiation of round spermatids into sperm. The gene Sly is present in multiple copies on the mouse Y chromosome and encodes a protein that is required for the epigenetic regulation of postmeiotic sex chromosome expression. The X chromosome carries two multicopy genes related to Sly: Slx and Slxl1. Here we investigate the role of Slx/Slxl1 using transgenically-delivered small interfering RNAs to disrupt their function. We show that Slx and Slxl1 are important for normal sperm differentiation and male fertility. Slx/Slxl1 deficiency leads to delay in spermatid elongation and sperm release. A high proportion of delayed spermatids are eliminated via apoptosis, with a consequent reduced sperm count. The remaining spermatozoa are abnormal with impaired motility and fertilizing abilities. Microarray analyses reveal that Slx/Slxl1 deficiency affects the metabolic processes occurring in the spermatid cytoplasm but does not lead to a global perturbation of sex chromosome expression; this is in contrast with the effect of Sly deficiency which leads to an up-regulation of X and Y chromosome genes. This difference may be due to the fact that SLX/SLXL1 are cytoplasmic while SLY is found in the nucleus and cytoplasm of spermatids.

Item Type: Article
DOI/Identification number: 10.1091/mbc.e10-07-0601
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Peter Ellis
Date Deposited: 19 Jan 2015 17:38 UTC
Last Modified: 09 Mar 2023 11:33 UTC
Resource URI: (The current URI for this page, for reference purposes)

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