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Chromosomal localization of GGA4 BACs containing QTL-linked microsatellites

Sazanov, Alexei A, Romanov, Michael N, Wardecka, B, Sazanova, Anna L, Korczak, M, Stekol'nikova, V A, Kozyreva, Alexandra A, Smirnov, Alexandr F, Dodgson, Jerry B (2004) Chromosomal localization of GGA4 BACs containing QTL-linked microsatellites. In: Hayes, Hélène, ed. Cytogenetic and Genome Research. 106 (1). 19 (Abstract P18). Karger Publishers (doi:10.1159/000078555) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:46521)

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Abstract

Meeting Abstract: P18. Shell thickness at age 53 weeks (ST53) and egg albumin weight at 33 weeks (AW33) differ by 7.5% and 3.3%, respectively, in Rhode Island Red and Polish Green-legged Partrigenous layers. Analysis of cosegregation in hybrids revealed linkage between ST53 and microsatellite MCW0114 (full linkage) and between AW33 and MCW0170 (1 cM). Chicken genetic map comparisons showed that ADL0241 and LEI0081 were the closest microsatellites to ST53 and AW33 QTLs, respectively. Chicken BAC-libraries 032- JF256-BI and 032-JF256-RI were screened with MCW0114, MCW0170, ADL0241 and LEI0081 and fifteen clones were identified and PCR-controlled. Biotin-labeled BACs were FISH-mapped to chicken mitotic chromosomes. Hybridization signals were detected within two regions: GGA4q11- 12 (BACs containing MCW0114 and ADL0241) and GGA4q21-22 (BACs containing MCW0170). This data will help positional cloning of AW33 and ST53 QTLs. This work was supported by grants PD02-1.4-175 (Russian Ministry of Education), 03-04-48060-, INTAS 04-2163 (Russian Foundation for Basic Research) and projects 99-35205-8566 and 2001-52100-11225 (USDA/ CSREES).

Item Type: Conference or workshop item (Paper)
DOI/Identification number: 10.1159/000078555
Uncontrolled keywords: genetics & heredity
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
S Agriculture > SF Animal culture
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Mike Romanov
Date Deposited: 06 Jan 2015 12:36 UTC
Last Modified: 16 Nov 2021 10:18 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/46521 (The current URI for this page, for reference purposes)
Romanov, Michael N: https://orcid.org/0000-0003-3584-4644
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