Alignment of the linkage map, physical map, and sequence of the chicken genome

Romanov, Michael N and Rondelli, Catherine M and Dodgson, Jerry B (2004) Alignment of the linkage map, physical map, and sequence of the chicken genome. In: International Plant and Animal Genome XII Conference, 10-14 January 2004, San Diego, CA, USA. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Abstract

Four BAC libraries constructed at Texas A&M University and at the Children's Hospital of Oakland Research Institute providing altogether ~28X coverage of the chicken genome have been screened with most of the suitable genes and markers that are found on the chicken genetic linkage map (Schmid et al., 2000). This has provided the means to align the chicken BAC contig physical map (Ren et al., 2003) with the linkage map and assign most contigs to their appropriate location on a chicken chromosome. It also has aided in assembly of the first draft sequence of the chicken genome. Our screening approach of choice is overgo hybridization, using four-dimensional pooling (36 probes at a time, one degree of redundancy), chosen from a matrix of 216 probes per screen. All BACs assigned to chicken genes and markers have been incorporated into a database available online (https://www.msu.edu/~dodgson/resources/resources.htm#bacdata). As of October 2003, the database includes nearly 5000 BAC assignments for 577 markers across almost all chicken chromosomes and linkage groups. On-going overgo hybridization is being used to do fine structure comparative mapping between the human and chicken genomes and to close gaps detected in the first round chicken sequence assembly. Overgo hybridization provides a cost-effective, high throughput method for integration of physical maps and linkage maps for domestic animal (and plant) species. Supported by the USDA/CSREES (Project numbers: 99-35205-8566 and 2001-52100-11225).

Item Type: Conference or workshop item (Poster)
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculties > Sciences > School of Biosciences > Biomedical Research Group
Depositing User: M. Romanov
Date Deposited: 05 Jan 2015 12:13 UTC
Last Modified: 27 Aug 2016 16:24 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/46433 (The current URI for this page, for reference purposes)
Romanov, Michael N: https://orcid.org/0000-0003-3584-4644
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