Protein pattern transfer for biosensor applications

Volcke, C., Gandhiraman, R.P., Basabe-Desmonts, L., Iacono, M., Gubala, V., Cecchet, F., Cafolla, A.A., Williams, D.E. (2009) Protein pattern transfer for biosensor applications. Biosensors and Bioelectronics, 25 (6). pp. 1295-1300. ISSN 0956-5663. (doi:10.1016/j.bios.2009.10.016) (KAR id:45242)

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Official URL: http://dx.doi.org/10.1016/j.bios.2009.10.016

Abstract

This paper presents a very simple, industrially scalable method for transferring a high-resolution, biologically active protein pattern from one substrate to another. We demonstrate the transfer of a protein pattern formed initially by microcontact printing from a silicon surface (to which this form of printing is applicable) onto a glass or polymer substrate, almost independently of the surface/bulk properties of the second substrate. A very thin, spin-coated layer of a sugar is used to preserve the structure and organization of proteins during the subsequent plasma deposition of a siloxane polymer, after which the protein pattern could simply be peeled off the silicon substrate and glued onto any other desired substrate. Â© 2009 Elsevier B.V. All rights reserved.

Item Type:	Article
DOI/Identification number:	10.1016/j.bios.2009.10.016
Additional information:	Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Biosens. Bioelectron. [Field not mapped to EPrints] C2 - 19900799 [Field not mapped to EPrints] AD - Biomedical Diagnostics Institute (BDI), Dublin City University, Glasnevin, Dublin 9, Ireland [Field not mapped to EPrints] AD - Research Centre in Physics of Matter and Radiation (PMR), University of Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium [Field not mapped to EPrints] AD - School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland [Field not mapped to EPrints] AD - MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Chemistry, University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled keywords:	Atomic force microscopy, Biosensors, Fluorescence, Microcontact printing, Protein pattern transfer, Biologically active proteins, Biosensor applications, Coated layers, High resolution, Micro contact printing, Polymer substrate, Protein pattern transfer, Protein patterns, Scalable methods, Silicon substrates, Silicon surfaces, Siloxane polymers, Atomic force microscopy, Biosensors, Chemical vapor deposition, Electron device manufacture, Fluorescence, Plasma deposition, Printing, Scalability, Substrates, Sugar (sucrose), Proteins, glass, glue, polymer, protein, silicon, siloxane, sugar, analytic method, article, atomic force microscopy, biosensor, immunoassay, physical chemistry, plasma, protein analysis, protein structure, protein transport, X ray photoelectron spectroscopy, Adsorption, Biocompatible Materials, Biosensing Techniques, Equipment Design, Equipment Failure Analysis, Immunoassay, Materials Testing, Protein Array Analysis, Protein Binding, Proteins, Reproducibility of Results, Sensitivity and Specificity, Surface Properties
Divisions:	Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User:	Vladimir Gubala
Date Deposited:	14 Dec 2017 21:19 UTC
Last Modified:	16 Nov 2021 10:18 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/45242 (The current URI for this page, for reference purposes)

University of Kent Author Information

Gubala, V..

Creator's ORCID:	https://orcid.org/0000-0001-6301-3632
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