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In silico reconstruction of chromosomal rearrangements and an avian ancestral karyotype

Romanov, Michael N, Farré-Belmonte, Marta, Lithgow, Pamela E, O'Connor, Rebecca, Fowler, Katie E, Larkin, Denis M, Griffin, Darren K. (2014) In silico reconstruction of chromosomal rearrangements and an avian ancestral karyotype. In: International Plant and Animal Genome XXII Conference, 11-16 January 2014, San Diego, CA, USA.

Abstract

The recent availability of whole genome assemblies for multiple bird species has changed the way we can detect and describe patterns of avian genome evolution. Coupled with conventional cytogenetic techniques, in silico comparison and alignment of bird chromosome assemblies offer exciting opportunities for reconstructing chromosomal rearrangement events that occurred in various avian and ancestral lineages. These can later be correlated with the variety of phenotypes observed in birds. We are testing this approach using the chromosome assemblies of zebra finch, turkey, duck, and green anole lizard (as an outgroup genome); analysis of further genomes is in progress. The approach first involves identification of multi-species homologous synteny blocks and reconstruction of a tentative avian ancestral genome from contiguous ancestral regions (CARs) using the multiple genome rearrangement and ancestors tool (MGRA). Secondly, manual comparative analysis of the CARs to reveal the most likely scenarios of inter- and intrachromosomal events in separate avian lineages is performed. Finally, we compare the rearrangement rates in each lineage. We frequently observe that species with the fewest number of chromosomal rearrangements exhibit phenotypes considered being ancestral for birds. In contrast, an increased rate of chromosomal rearrangements often correlates with higher degree of phenotypic variation, diversification, and adaptation.

Item Type: Conference or workshop item (Poster)
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculties > Sciences > School of Biosciences > Biomedical Research Group
Depositing User: M. Romanov
Date Deposited: 18 Dec 2013 10:15 UTC
Last Modified: 29 May 2019 11:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/37651 (The current URI for this page, for reference purposes)
Romanov, Michael N: https://orcid.org/0000-0003-3584-4644
Farré-Belmonte, Marta: https://orcid.org/0000-0001-9170-5767
O'Connor, Rebecca: https://orcid.org/0000-0002-4270-970X
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