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A BAC-based physical map of the chicken genome

Ren, Chengwei, Lee, Mi-Kyung, Yan, Bo, Ding, Kejiao, Cox, Bettye, Romanov, Michael N, Price, Jennifer A, Dodgson, Jerry B, Zhang, Hongbin (2003) A BAC-based physical map of the chicken genome. Genome Research, 13 (12). pp. 2754-2758. ISSN 1088-9051. (doi:10.1101/gr.1499303)

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Abstract

A genome-wide physical map constructed with bacterial artificial chromosomes (BACs) is an essential component in linking phenotypic traits to the responsible genetic variation in the genomes of plants and animals. We have constructed a physical map of the chicken genome from 57,091 BACs (7.9-fold haploid genome coverage) by restriction fingerprint analysis using high-resolution polyacrylamide gel electrophoresis. The physical map consists of 2331 overlapping BAC contigs and is estimated to span 1510 Mb in physical length. BAC contigs were verified manually and by screening the BACs with 367 DNA markers. A total of 361 of the contigs have been anchored to the existing chicken genetic map. This map represents the first genome-wide, BAC-based physical map of the chicken genome. It provides a powerful platform for many areas of chicken genomics, including targeted marker development, fine mapping of genes and QTL alleles, positional cloning, analysis of avian genome organization and evolution, chicken-mammalian comparative genomics, and large-scale genome sequencing.

Item Type: Article
DOI/Identification number: 10.1101/gr.1499303
Uncontrolled keywords: chicken genome; contigs; model; genes
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculties > Sciences > School of Biosciences > Biomedical Research Group
Depositing User: M. Romanov
Date Deposited: 12 Dec 2013 16:57 UTC
Last Modified: 29 May 2019 11:40 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/37566 (The current URI for this page, for reference purposes)
Romanov, Michael N: https://orcid.org/0000-0003-3584-4644
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