Romanov, Michael N, Price, Jennifer A, Dodgson, Jerry B (2003) Integration of animal linkage and BAC contig maps using overgo hybridization. Cytogenetic and Genome Research, 102 (1-4). pp. 277-281. ISSN 1424-8581. E-ISSN 1424-859X. (doi:10.1159/000075763) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:37564)
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Official URL: https://doi.org/10.1159/000075763 |
Abstract
The alignment of genome linkage maps, defined primarily by segregation of sequence-tagged site (STS) markers, with BAC contig physical maps and full genome sequences requires high throughput mechanisms to identify BAC clones that contain specific STS. A powerful technique for this purpose is multi-dimensional hybridization of "overgo" probes. The probes are chosen from available STS sequence data by selecting unique probe sequences that have a common melting temperature. We have hybridized sets of 216 overgo probes in subset pools of 36 overgos at a time to filter-spotted chicken BAC clone arrays. A four-dimensional pooling strategy, including one degree of redundancy, has been employed. This requires 24 hybridizations to completely assign BACs for all 216 probes. Results to date are consistent with about a 10% failure rate in overgo probe design and a 15-20% false negative detection rate within a group of 216 markers. Three complete rounds of overgo hybridization, each to sets of about 39,000 BACs (either BamHI or EcoRI partial digest inserts) generated a total of 1853 BAC alignments for 517 mapped chicken genome STS markers. These data are publicly available, and they have been used in the assembly of a first generation BAC contig map of the chicken genome.
Item Type: | Article |
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DOI/Identification number: | 10.1159/000075763 |
Uncontrolled keywords: | chicken genome; genes |
Subjects: |
Q Science > QH Natural history > QH426 Genetics S Agriculture > SF Animal culture |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Signature Themes: | Food Systems, Natural Resources and Environment |
Depositing User: | Mike Romanov |
Date Deposited: | 12 Dec 2013 17:07 UTC |
Last Modified: | 08 Sep 2023 15:56 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/37564 (The current URI for this page, for reference purposes) |
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