Integration of the cytogenetic and physical maps of chicken chromosome 17

Romanov, Michael N and Daniels, Laura and Dodgson, Jerry B and Delany, Mary E (2005) Integration of the cytogenetic and physical maps of chicken chromosome 17. Chromosome Research, 13 (2). pp. 215-222. ISSN 0967-3849. E-ISSN 1573-6849. (doi:https://doi.org/10.1007/s10577-005-1506-3) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1007/s10577-005-1506-3

Abstract

The chicken genome, like those of most avian species, contains numerous microchromosomes that cannot be distinguished by size alone. Unique properties attributed to the microchromosomes include high GC content and gene density, and an enhanced recombination rate. Previously, microchromosome GGA 17 was shown to align with the consensus genetic linkage group E41W17, and bacterial artificial chromosome (BAC) clones containing E41W17 markers were isolated and assigned on the physical BAC map as well as the recently assembled draft chicken genome sequence. For this study, these same BACs were utilized as probes for fluorescence in-situ hybridization (FISH) to develop the GGA 17 cytogenetic map. Here we detail the chromosome order of ten BAC DNAs, thereby deriving a cytogenetic map of GGA 17 that is simultaneously integrated with both the linkage map and genome sequence. The location of the FISH probes together with the morphological appearance of the chromosome suggested that GGA 17 is an acrocentric chromosome whose cytogenetic map orientation is reversed from that currently indicated by the linkage map and draft genome sequence. The reversed orientation and the centromere location of GGA 17 were confirmed experimentally by dual-colour FISH hybridization using terminal BACs and the centromere-specific CNM oligonucleotide as probes. An advantage of this cyto-genomic approach is the improved alignment of the sequence and linkage maps with cytogenetic features such as the centromere, telomeres, p and q arms, and staining patterns indicating GC versus AT content.

Item Type: Article
Uncontrolled keywords: BAC; chicken; cytogenetics; fluorescence in-situ hybridization; Gallus; genetic linkage; genomics; repetitive DNA
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculties > Sciences > School of Biosciences > Biomedical Research Group
Depositing User: M. Romanov
Date Deposited: 12 Dec 2013 17:23 UTC
Last Modified: 26 Jan 2015 13:49 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/37555 (The current URI for this page, for reference purposes)
Romanov, Michael N: https://orcid.org/0000-0003-3584-4644
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