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Chromosomal localization of seven HSA3q13→q23 NotI linking clones on chicken microchromosomes: orthology of GGA14 and GGA15 to a gene-rich region of HSA3

Sazanov, Alexei A, Sazanova, Anna L, Stekol'nikova, V A, Kozyreva, Alexandra A, Romanov, Michael N, Malewski, T, Smirnov, Alexandr F (2005) Chromosomal localization of seven HSA3q13→q23 NotI linking clones on chicken microchromosomes: orthology of GGA14 and GGA15 to a gene-rich region of HSA3. Cytogenetic and Genome Research, 111 (2). pp. 128-133. ISSN 1424-8581. E-ISSN 1424-859X. (doi:10.1159/000086381) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:37553)

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Double-color fluorescence in situ hybridization was performed on chicken chromosomes using seven unique clones from the human chromosome 3-specific NotI linking libraries. Six of them (NL1-097, NL2-092, NL2-230, NLM-007, NLM-118, and NLM-196) were located on the same chicken microchromosome and NL1-290 on another. Two chicken microchromosome GGA15-specific BAC clones, JE024F14 containing the IGVPS gene and JE020G17 containing the ALDH1A1 gene, were cytogenetically mapped to the same microchromosome that carried the six NotI linking clones, allowing identification of this chromosome as GGA15. Two GGA14-specific clones, JE027C23 and JE014E08 containing the HBA gene cluster, were co-localized on the same microchromosome as NL1-290, suggesting that this chromosome was GGA14. The results indicated that the human chromosomal region HSA3q13-->q23 is likely to be orthologous to GGA15 and GGA14. The breakpoint of evolutionary conservation of human and chicken chromosomes was detected on HSA3q13.3-->q23 between NL1-290, on the one hand, and six other NotI clones, on the other hand. Considering the available chicken-human comparative mapping data, another breakpoint appears to exist between the above NotI loci and four other genes, TFRC, EIF4A2, SKIL and DHX36 located on HSA3q24-->qter and GGA9. Based on human sequences within the NotI clones, localization of the six new chicken coding sequences orthologous to the human/rodent genes was suggested to be on GGA15 and one on GGA14. Microchromosomal location of seven NotI clones from the HSA3q21 T-band region can be considered as evidence in support of our hypothesis about the functional analogy of mammalian T-bands and avian microchromosomes.

Item Type: Article
DOI/Identification number: 10.1159/000086381
Uncontrolled keywords: in-situ hybridization; human genome; CPG islands; evolution; libraries; map; human chromosome 3; construction; integration; linkage
Subjects: Q Science > QH Natural history > QH426 Genetics
S Agriculture > SF Animal culture
Divisions: Divisions > Division of Natural Sciences > Biosciences
Signature Themes: Food Systems, Natural Resources and Environment
Depositing User: Mike Romanov
Date Deposited: 12 Dec 2013 17:24 UTC
Last Modified: 08 Sep 2023 16:53 UTC
Resource URI: (The current URI for this page, for reference purposes)

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