Investigation of pseudoautosomal and bordering regions in avian Z and W chromosomes with the use of large insert genomic BAC clones

Blagoveshchenski?, I Yu and Sazanova, Anna L and Stekol'nikova, V A and Fomichev, K A and Barkova, O Yu and Romanov, Michael N and Sazanov, Alexei A (2011) Investigation of pseudoautosomal and bordering regions in avian Z and W chromosomes with the use of large insert genomic BAC clones. Russian Journal of Genetics, 47 (3). pp. 272-278. ISSN 1022-7954. E-ISSN 1608-3369. (doi:https://doi.org/10.1134/S1022795411020050) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1134/S1022795411020050

Abstract

To study pseudoautosomal and bordering regions in the avian Z and W chromosomes, we used seven BAC clones from genomic libraries as DNA probes of fragments of different gametologs of the ATP5A1 gene located close to the proximal border of the pseudoautosomal region (PAR) of sex chromosomes of domestic chicken and Japanese quail. Localization of BAC clones TAM31-b100C09, TAM31-b99N01, TAM31-b27P16, and TAM31-b95L18 in the short arm of Z chromosomes of domestic chicken and Japanese quail (region Zp23-p22) and localization of the BAC clones CHORI-261-CH46G16, CHORI-261-CH33F10, and CHORI-261-CH64F22 on W chromosomes of these species and in the short arm of Z chromosomes (region Zp23-p22) were determined by fluorescence in situ hybridization with the use of W-specific probes. The difference in the localization of the BAC clones on the Z and W chromosomes is probably explained by divergence of the nucleotide sequences of different sex chromosomes located beyond the pseudoautosomal region.

Item Type: Article
Uncontrolled keywords: evolutionary strata; gallus-domesticus; sex-chromosomes; chicken; genes; quail; FISH; map; DNA
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculties > Sciences > School of Biosciences > Biomedical Research Group
Depositing User: M. Romanov
Date Deposited: 12 Dec 2013 16:13 UTC
Last Modified: 22 Aug 2018 09:32 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/37551 (The current URI for this page, for reference purposes)
Romanov, Michael N: https://orcid.org/0000-0003-3584-4644
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