Cross-species overgo hybridization and comparative physical mapping within avian genomes

Romanov, Michael N, Dodgson, Jerry B (2006) Cross-species overgo hybridization and comparative physical mapping within avian genomes. Animal Genetics, 37 (4). pp. 397-399. ISSN 0268-9146. (doi:10.1111/j.1365-2052.2006.01463.x) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:37548)

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Official URL: https://doi.org/10.1111/j.1365-2052.2006.01463.x

Abstract

The chicken genome sequence facilitates comparative genomics within other avian species. We performed cross-species hybridizations using overgo probes designed from chicken genomic and zebra finch expressed sequence tags (ESTs) to turkey and zebra finch BAC libraries. As a result, 3772 turkey BACs were assigned to 336 markers or genes, and 1662 zebra finch BACs were assigned to 164 genes. As expected, cross-hybridization was more successful with overgos within coding sequences than within untranslated region, intron or flanking sequences and between chicken and turkey, when compared with chicken-zebra finch or zebra finch-turkey cross-hybridization. These data contribute to the comparative alignment of avian genome maps using a 'one sequence, multiple genomes' strategy.

Item Type:	Article
DOI/Identification number:	10.1111/j.1365-2052.2006.01463.x
Uncontrolled keywords:	avian genomes; comparative mapping; cross-species hybridization; overgo probes
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QL Zoology
Divisions:	Divisions > Division of Natural Sciences > Biosciences
Signature Themes:	Food Systems, Natural Resources and Environment
Funders:	United States Department of Agriculture (https://ror.org/01na82s61) National Institutes of Health (https://ror.org/01cwqze88)
Depositing User:	Mike Romanov
Date Deposited:	12 Dec 2013 16:26 UTC
Last Modified:	08 Sep 2023 15:11 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/37548 (The current URI for this page, for reference purposes)

University of Kent Author Information

Romanov, Michael N.

Creator's ORCID:	https://orcid.org/0000-0003-3584-4644
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