Nuclear pore components are involved in the transcriptional regulation of dosage compensation in Drosophila.

Mendjan, Sascha, Taipale, Mikko, Kind, Jop H, Holz, Herbert, Gebhardt, Philipp, Schelder, Malgorzata, Vermeulen, Michiel, Buscaino, Alessia, Duncan, Kent, Mueller, Juerg, and others. (2006) Nuclear pore components are involved in the transcriptional regulation of dosage compensation in Drosophila. Molecular Cell, 21 (6). pp. 811-823. ISSN 1097-2765. (doi:10.1016/j.molcel.2006.02.007) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:34597)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL: http://dx.doi.org/10.1016/j.molcel.2006.02.007

Abstract

Dosage compensation in Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from Drosophila embryos, Schneider cells, and human HeLa cells. We find a stable association of the histone H4 lysine 16-specific acetyltransferase MOF with the RNA/protein containing MSL complex as well as with an evolutionary conserved complex. We show that the MSL complex interacts with several components of the nuclear pore, in particular Mtor/TPR and Nup153. Strikingly, knockdown of Mtor or Nup153 results in loss of the typical MSL X-chromosomal staining and dosage compensation in Drosophila male cells but not in female cells. These results reveal an unexpected physical and functional connection between nuclear pore components and chromatin regulation through MSL proteins, highlighting the role of nucleoporins in gene regulation in higher eukaryotes.

Item Type:	Article
DOI/Identification number:	10.1016/j.molcel.2006.02.007
Uncontrolled keywords:	DNA; DEVBIO
Subjects:	Q Science
Divisions:	Divisions > Division of Natural Sciences > Biosciences
Depositing User:	Alessia Buscaino
Date Deposited:	12 Jul 2013 08:45 UTC
Last Modified:	05 Nov 2024 10:17 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/34597 (The current URI for this page, for reference purposes)

University of Kent Author Information

Buscaino, Alessia.

Creator's ORCID:	https://orcid.org/0000-0002-1704-3168
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