Storbeck, Sonja, Rolfes, Sarah, Raux-Deery, Evelyne, Warren, Martin J., Jahn, Dieter, Layer, Gunhild (2010) A Novel Pathway for the Biosynthesis of Heme in Archaea: Genome-Based Bioinformatic Predictions and Experimental Evidence. Archaea, 2010 (1). pp. 1-15. ISSN 1472-3646. (doi:10.1155/2010/175050) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:34342)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: http://dx.doi.org/10.1155/2010/175050 |
Abstract
Heme is an essential prosthetic group for many proteins involved in fundamental biological processes in all three domains of life. In Eukaryota and Bacteria heme is formed via a conserved and well-studied biosynthetic pathway. Surprisingly, in Archaea heme biosynthesis proceeds via an alternative route which is poorly understood. In order to formulate a working hypothesis for this novel pathway, we searched 59 completely sequenced archaeal genomes for the presence of gene clusters consisting of established heme biosynthetic genes and colocalized conserved candidate genes. Within the majority of archaeal genomes it was possible to identify such heme biosynthesis gene clusters. From this analysis we have been able to identify several novel heme biosynthesis genes that are restricted to archaea. Intriguingly, several of the encoded proteins display similarity to enzymes involved in heme d1 biosynthesis. To initiate an experimental verification of our proposals two Methanosarcina barkeri proteins predicted to catalyze the initial steps of archaeal heme biosynthesis were recombinantly produced, purified, and their predicted enzymatic functions verified.
Item Type: | Article |
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DOI/Identification number: | 10.1155/2010/175050 |
Subjects: | Q Science |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | Lin Riches |
Date Deposited: | 25 Jun 2013 14:29 UTC |
Last Modified: | 05 Nov 2024 10:17 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/34342 (The current URI for this page, for reference purposes) |
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