Valproic acid induces extracellular signal-regulated kinase 1/2 activation and inhibits apoptosis in endothelial cells.

Michaelis, Martin, Suhan, Tatyana, Michaelis, U. Ruth, Beek, K, Rothweiler, Florian, Tausch, L., Werz, O., Eikel, Daniel, Zörnig, M., Nau, Heinz, and others. (2006) Valproic acid induces extracellular signal-regulated kinase 1/2 activation and inhibits apoptosis in endothelial cells. Cell Death and Differentiation, 13 (3). pp. 446-53. ISSN 1350-9047. (doi:10.1038/sj.cdd.4401759) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:34105)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL: http://dx.doi.org/10.1038/sj.cdd.4401759

Abstract

The histone deacetylase (HDAC) inhibitor valproic acid (VPA) was recently shown to inhibit angiogenesis, but displays no toxicity in endothelial cells. Here, we demonstrate that VPA increases extracellular signal-regulated kinase 1/2 (ERK 1/2) phosphorylation in human umbilical vein endothelial cells (HUVEC). The investigation of structurally modified VPA derivatives revealed that the induction of ERK 1/2 phosphorylation is not correlated to HDAC inhibition. PD98059, a pharmacological inhibitor of the mitogen-activated protein kinase kinase 1/2, prevented the VPA-induced ERK 1/2 phosphorylation. In endothelial cells, ERK 1/2 phosphorylation is known to promote cell survival and angiogenesis. Our results showed that VPA-induced ERK 1/2 phosphorylation in turn causes phosphorylation of the antiapoptotic protein Bcl-2 and inhibits serum starvation-induced HUVEC apoptosis and cytochrome c release from the mitochondria. Moreover, the combination of VPA with PD98059 synergistically inhibited angiogenesis in vitro and in vivo.

Item Type:	Article
DOI/Identification number:	10.1038/sj.cdd.4401759
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	Divisions > Division of Natural Sciences > Biosciences
Depositing User:	Martin Michaelis
Date Deposited:	05 Jun 2013 20:44 UTC
Last Modified:	16 Nov 2021 10:11 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/34105 (The current URI for this page, for reference purposes)

University of Kent Author Information

Michaelis, Martin.

Creator's ORCID:	https://orcid.org/0000-0002-5710-5888
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