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Production of Amino-Terminally Acetylated Recombinant Proteins in E. coli.

Johnson, Matthew and Geeves, Michael A. and Mulvihill, Daniel P. (2013) Production of Amino-Terminally Acetylated Recombinant Proteins in E. coli. In: Hake, Sandra and Janzen, Christian, eds. Protein Acetylation. Methods and Protocols, 981 . Humana Press, pp. 193-200. ISBN 978-1-62703-304-6. (doi:10.1007/978-1-62703-305-3_15) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:33930)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1007/978-1-62703-305-3_15

Abstract

The majority of proteins in eukaryote cells are subjected to amino-terminal acetylation. This co-translational modification can affect the stability of a protein and also regulate its biological function. Amino-terminally acetylated recombinant proteins cannot be produced using prokaryote expression systems, such as E. coli, as these cells lack the appropriate N-?-terminal acetyltransferase complexes. Here we describe a simple protocol that allows the recombinant expression and purification of NatB-dependent amino-terminally acetylated proteins from E. coli.

Item Type: Book section
DOI/Identification number: 10.1007/978-1-62703-305-3_15
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Susan Davies
Date Deposited: 20 May 2013 14:16 UTC
Last Modified: 06 Feb 2020 04:07 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/33930 (The current URI for this page, for reference purposes)
Geeves, Michael A.: https://orcid.org/0000-0002-9364-8898
Mulvihill, Daniel P.: https://orcid.org/0000-0003-2502-5274
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