Production of Amino-Terminally Acetylated Recombinant Proteins in E. coli.

Johnson, Matthew and Geeves, Michael A. and Mulvihill, Daniel P. (2013) Production of Amino-Terminally Acetylated Recombinant Proteins in E. coli. In: Hake, Sandra and Janzen, Christian, eds. Protein Acetylation. Methods and Protocols, 981 . Humana Press, pp. 193-200. ISBN 978-1-62703-304-6. (doi:https://doi.org/10.1007/978-1-62703-305-3_15) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1007/978-1-62703-305-3_15

Abstract

The majority of proteins in eukaryote cells are subjected to amino-terminal acetylation. This co-translational modification can affect the stability of a protein and also regulate its biological function. Amino-terminally acetylated recombinant proteins cannot be produced using prokaryote expression systems, such as E. coli, as these cells lack the appropriate N-α-terminal acetyltransferase complexes. Here we describe a simple protocol that allows the recombinant expression and purification of NatB-dependent amino-terminally acetylated proteins from E. coli.

Item Type: Book section
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Sue Davies
Date Deposited: 20 May 2013 14:16 UTC
Last Modified: 06 Nov 2014 11:54 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/33930 (The current URI for this page, for reference purposes)
  • Depositors only (login required):