Johnson, Matthew and Geeves, Michael A. and Mulvihill, Daniel P. (2013) Production of Amino-Terminally Acetylated Recombinant Proteins in E. coli. In: Hake, Sandra and Janzen, Christian, eds. Protein Acetylation. Methods and Protocols, 981 . Humana Press, pp. 193-200. ISBN 978-1-62703-304-6. (doi:10.1007/978-1-62703-305-3_15) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:33930)
| The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
| Official URL: http://dx.doi.org/10.1007/978-1-62703-305-3_15 |
|
Abstract
The majority of proteins in eukaryote cells are subjected to amino-terminal acetylation. This co-translational modification can affect the stability of a protein and also regulate its biological function. Amino-terminally acetylated recombinant proteins cannot be produced using prokaryote expression systems, such as E. coli, as these cells lack the appropriate N-?-terminal acetyltransferase complexes. Here we describe a simple protocol that allows the recombinant expression and purification of NatB-dependent amino-terminally acetylated proteins from E. coli.
| Item Type: | Book section |
|---|---|
| DOI/Identification number: | 10.1007/978-1-62703-305-3_15 |
| Subjects: | Q Science |
| Institutional Unit: | Schools > School of Natural Sciences > Biosciences |
| Former Institutional Unit: |
Divisions > Division of Natural Sciences > Biosciences
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| Depositing User: | Susan Davies |
| Date Deposited: | 20 May 2013 14:16 UTC |
| Last Modified: | 20 May 2025 09:17 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/33930 (The current URI for this page, for reference purposes) |
University of Kent Author Information
Geeves, Michael A..
| Creator's ORCID: |
 https://orcid.org/0000-0002-9364-8898
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|---|---|
| CReDIT Contributor Roles: |
Mulvihill, Daniel P..
| Creator's ORCID: |
https://orcid.org/0000-0003-2502-5274
|
|---|---|
| CReDIT Contributor Roles: |
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