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Measuring protein reduction potentials using 15N HSQC NMR spectroscopy

Taylor, Samantha L., Crawley-Snowdon, Harriet, Wagstaff, Jane L., Rowe, Michelle L., Shepherd, Mark, Williamson, Richard A., Howard, Mark J. (2013) Measuring protein reduction potentials using 15N HSQC NMR spectroscopy. Chemical Communications, 49 (18). pp. 1847-1849. ISSN 1359-7345. (doi:10.1039/c3cc38952a)

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Abstract

NMR spectroscopy was used to measure reduction potentials of four redox proteins by following multiple 15N HSQC protein resonances across a titration series using mixtures of oxidised and reduced glutathione. Results for PDI a, PDI ab and DsbA agree with the literature and our result for ERp18 confirms this protein as an oxidoreductase of comparable or greater reducing strength than PDI a.

Item Type: Article
DOI/Identification number: 10.1039/c3cc38952a
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Faculties > Sciences > School of Biosciences > Protein Science Group
Depositing User: M.J. Howard
Date Deposited: 28 Mar 2013 11:43 UTC
Last Modified: 29 May 2019 10:05 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/33499 (The current URI for this page, for reference purposes)
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