Alberini, Isabella, Del Tordello, Elena, Fasolo, Alba, Temperton, Nigel J., Galli, Grazia, Gentile, Chiara, Montomoli, Emanuele, Hilbert, Anne K., Banzhoff, Angelika, Del Giudice, Giuseppe, and others. (2009) Pseudoparticle neutralization is a reliable assay to measure immunity and cross-reactivity to H5N1 influenza viruses. Vaccine, 27 (43). pp. 5998-6003. ISSN 0264-410X. (doi:10.1016/j.vaccine.2009.07.079) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:29400)
| The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
| Official URL: http://dx.doi.org/10.1016/j.vaccine.2009.07.079 |
Abstract
The standard serological methods present limitations for the measurement of immunity against H5N1 influenza strains. The hemagglutination inhibition (HI) assay lacks sensitivity and requires standardization, while the viral micro-neutralization (MN) assay needs handling of live virus. We produced pseudoparticles expressing hemagglutinin from clades 1 or 2 H5N1 in order to measure neutralizing antibodies in human sera after prime-boost vaccination with plain or MF59-adjuvanted H5N1 clade 1 subunit vaccines. Titers measured by pseudoparticle neutralization (PPN) assay significantly correlated with those measured by HI, single radial haemolysis or MN, with a PPN titer of 1:357 corresponding to an MN titer of 1:80. Notably, results from the PPN assay, confirm that MF59-H5N1 vaccine induces potent and long-lasting neutralizing antibody responses not only against the vaccine strain, but also against several heterologous clade 2 strains. Overall, the PPN assay represents a valid alternative to conventional serological methods for the evaluation of H5N1 vaccine immunogenicity. © 2009 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.1016/j.vaccine.2009.07.079 |
| Uncontrolled keywords: | Avian influenza virus, Cross-clade neutralization, H5N1, Pandemic vaccine, Pseudoparticle neutralization assay, Serological methods, avian influenza vaccine, n acetylmuramylalanyl dextro isoglutaminylalanyl dipalmitoylphosphatidylethanolamine, neutralizing antibody, virus hemagglutinin, article, avian influenza, blood, blood sampling, cross reaction, hemagglutination inhibition test, hemolysis, human, immunity, Influenza virus A H5N1, priority journal, serodiagnosis, virus strain, virus titration, Adult, Antibodies, Viral, Cross Reactions, Hemagglutination Inhibition Tests, Hemagglutinin Glycoproteins, Influenza Virus, Humans, Influenza A Virus, H5N1 Subtype, Influenza Vaccines, Influenza, Human, Neutralization Tests |
| Subjects: | Q Science > QR Microbiology > QR355 Virology |
| Divisions: | Divisions > Division of Natural Sciences > Medway School of Pharmacy |
| Depositing User: | Nigel Temperton |
| Date Deposited: | 02 Aug 2012 06:51 UTC |
| Last Modified: | 05 Nov 2024 10:10 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/29400 (The current URI for this page, for reference purposes) |
University of Kent Author Information
Temperton, Nigel J..
| Creator's ORCID: |
 https://orcid.org/0000-0002-7978-3815
|
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