Johnson, Matthew, Couton, Arthur T., Geeves, Michael A., Mulvihill, Daniel P. (2010) Targeted amino-terminal acetylation of recombinant proteins in E. coli. PLoS ONE, 5 (12). e15801. ISSN 1932-6203. (doi:10.1371/journal.pone.0015801) (KAR id:26186)
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| Official URL: http://dx.plos.org/10.1371/journal.pone.0015801 |
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Abstract
One major limitation in the expression of eukaryotic proteins in bacteria is an inability to post-translationally modify the expressed protein. Amino-terminal acetylation is one such modification that can be essential for protein function. By co- expressing the fission yeast NatB complex with the target protein in E.coli, we report a simple and widely applicable method for the expression and purification of functional N-terminally acetylated eukaryotic proteins.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.1371/journal.pone.0015801 |
| Subjects: |
Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology |
| Divisions: | Divisions > Division of Natural Sciences > Biosciences |
| Funders: |
Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982)
Wellcome Trust (https://ror.org/029chgv08) |
| Depositing User: | Daniel Mulvihill |
| Date Deposited: | 30 Dec 2010 18:10 UTC |
| Last Modified: | 12 Jul 2022 10:40 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/26186 (The current URI for this page, for reference purposes) |
| Geeves, Michael A.: |  https://orcid.org/0000-0002-9364-8898 |
| Mulvihill, Daniel P.: | https://orcid.org/0000-0003-2502-5274 |
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