Johnson, Matthew, Couton, Arthur T., Geeves, Michael A., Mulvihill, Daniel P. (2010) Targeted amino-terminal acetylation of recombinant proteins in E. coli. PLoS ONE, 5 (12). e15801. ISSN 1932-6203. (doi:10.1371/journal.pone.0015801) (KAR id:26186)
PDF (Johnson et al)
Language: English |
|
Download this file (PDF/389kB) |
Preview |
Request a format suitable for use with assistive technology e.g. a screenreader | |
Official URL: http://dx.plos.org/10.1371/journal.pone.0015801 |
Abstract
One major limitation in the expression of eukaryotic proteins in bacteria is an inability to post-translationally modify the expressed protein. Amino-terminal acetylation is one such modification that can be essential for protein function. By co- expressing the fission yeast NatB complex with the target protein in E.coli, we report a simple and widely applicable method for the expression and purification of functional N-terminally acetylated eukaryotic proteins.
Item Type: | Article |
---|---|
DOI/Identification number: | 10.1371/journal.pone.0015801 |
Subjects: |
Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Funders: |
Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982)
Wellcome Trust (https://ror.org/029chgv08) |
Depositing User: | Daniel Mulvihill |
Date Deposited: | 30 Dec 2010 18:10 UTC |
Last Modified: | 12 Jul 2022 10:40 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/26186 (The current URI for this page, for reference purposes) |
- Link to SensusAccess
- Export to:
- RefWorks
- EPrints3 XML
- BibTeX
- CSV
- Depositors only (login required):