Selective perturbation of the myosin recovery stroke by point mutations at the base of the lever arm affects ATP hydrolysis and phosphate release

Malnasi-Csizmadia, András, Toth, Judit, Pearson, David S., Hetenyi, Csaba, Nyitray, László, Geeves, Michael A., Bagshaw, Clive R, Kovacs, Mihály (2007) Selective perturbation of the myosin recovery stroke by point mutations at the base of the lever arm affects ATP hydrolysis and phosphate release. Journal of Biological Chemistry, 282 (24). pp. 17658-17664. ISSN 0021-9258. (doi:10.1074/jbc.M701447200) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:2593)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL: http://dx.doi.org/10.1074/jbc.M701447200

Abstract

After ATP binding the myosin head undergoes a large structural rearrangement called the recovery stroke. This transition brings catalytic residues into place to enable ATP hydrolysis, and at the same time it causes a swing of the myosin lever arm into a primed state, which is a prerequisite for the power stroke. By introducing point mutations into a subdomain interface at the base of the myosin lever arm at positions Lys(84) and Arg(704), we caused modulatory changes in the equilibrium constant of the recovery stroke, which we could accurately resolve using the fluorescence signal of single tryptophan Dictyostelium myosin II constructs. Our results shed light on a novel role of the recovery stroke: fine-tuning of this reversible equilibrium influences the functional properties of myosin through controlling the effective rates of ATP hydrolysis and phosphate release.

Item Type:	Article
DOI/Identification number:	10.1074/jbc.M701447200
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology
Divisions:	Divisions > Division of Natural Sciences > Biosciences
Depositing User:	Suzanne Duffy
Date Deposited:	31 Mar 2008 18:01 UTC
Last Modified:	05 Nov 2024 09:33 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/2593 (The current URI for this page, for reference purposes)

University of Kent Author Information

Geeves, Michael A..

Creator's ORCID:	https://orcid.org/0000-0002-9364-8898
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