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Direct detection of Listeria monocytogenes from milk by magnetic based DNA isolation and PCR

Amagliani, G., Brandi, G., Omiccioli, E., Magnani, Mauro, Bruce, Ian J., Casiere, A. (2004) Direct detection of Listeria monocytogenes from milk by magnetic based DNA isolation and PCR. Food Microbiology, 21 (5). pp. 597-603. ISSN 0740-0020. (doi:10.1016/j.fm.2003.10.008) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:22811)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1016/j.fm.2003.10.008

Abstract

Two nucleic acid-based methods for rapid and sensitive detection of the foodborne pathogen Listeria monocytogenes in milk were developed in this study. These methods rely on paramagnetic nanoparticle-based isolation of bacterial DNA directly from milk and subsequent PCR with selective primers for the listeriolysin O (hlyA) gene. The hlyA specific product was reproducibly detected and showed a sensitivity of 10 cfu ml(-1). The magnetic-based system had a sensitivity 10-fold higher than that of commercially available column devices. The detection limit of both methods is sufficient for direct detection of L. monocytogenes DNA in milk avoiding the enrichment culturing step, reducing the time necessary to obtain results from samples to 7 h rather than the 5-day minimum required for the standard procedure. The methods developed are suitable for automation.

Item Type: Article
DOI/Identification number: 10.1016/j.fm.2003.10.008
Uncontrolled keywords: Listeria monocytogenes; hlyA; paramagnetic nanoparticles; milk
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Suzanne Duffy
Date Deposited: 11 Sep 2009 13:22 UTC
Last Modified: 05 Nov 2024 10:02 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/22811 (The current URI for this page, for reference purposes)

University of Kent Author Information

Bruce, Ian J..

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