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The Volume Changes Associated with the Operation of the Simple Transporter

Hall, Andrew C., Jarvis, Simon M. (1992) The Volume Changes Associated with the Operation of the Simple Transporter. Biochimica Et Biophysica Acta, 1105 (2). pp. 300-306. ISSN 0006-3002. (doi:10.1016/0005-2736(92)90208-4) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:22602)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1016/0005-2736(92)90208-4

Abstract

The effects of hydrostatic pressure (0.1-50 MPa) on uridine transport mediated by the 'simple' facilitated nucleoside transporter of guinea-pig and human erythrocytes have been studied in an attempt to identify the volume changes which occur during transport. Pressure inhibited the zero-trans (influx or efflux) mode of uridine transport in guinea-pig cells significantly more (about 2.2-X) than equilibrium exchange. The equilibrium binding of H-3-nitrobenzylthioinosine, a potent specific inhibitor of nucleoside transport, to human red cells and ghosts, was not significantly altered by pressure suggesting that the permeation site was unperturbed. Thus pressure inhibited the transporter primarily by preventing the volume increase associated with the translocation step. Furthermore, the return of the 'empty' transporter was found to be rate-limiting because it required a larger increase in volume than when the transporter was loaded with substrate.

Item Type: Article
DOI/Identification number: 10.1016/0005-2736(92)90208-4
Uncontrolled keywords: Nucleoside Transport; Nitrobenzylthioinosine; Hydrostatic Pressure; Erythrocyte; Membrane Transport
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: P. Ogbuji
Date Deposited: 01 Oct 2009 08:35 UTC
Last Modified: 16 Nov 2021 10:01 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/22602 (The current URI for this page, for reference purposes)

University of Kent Author Information

Jarvis, Simon M..

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