Presence and Coding Properties of 2'-O-Methyl-5-Carbamoylmethyluridine (Ncm5um) in the Wobble Position of the Anticodon of Trnaleu (U-Asterisk-Aa) From Brewers-Yeast

Glasser, Anne-Lise and Eladlouni, Chakib and Keith, Gerard and Sochacka, Elzbieta and Malkiewicz, Andrzej and Santos, Manuel and Tuite, Mick F. and Desgres, Jean (1992) Presence and Coding Properties of 2'-O-Methyl-5-Carbamoylmethyluridine (Ncm5um) in the Wobble Position of the Anticodon of Trnaleu (U-Asterisk-Aa) From Brewers-Yeast. FEBS Letters, 314 (3). pp. 381-385. ISSN 0014-5793. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1016/0014-5793(92)81510-S

Abstract

The unknown modified nucleoside U* has been isolated by enzymatic and HPLC protocols from tRNA(Leu) (U*AA) recently discovered in brewer's yeast. The pure U* nucleoside has been characterized by electron impact mass spectroscopy, and comparison of its chromatographic and UV-absorption properties with those of appropriate synthetic compounds. The structure of U* was established as 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um). The yeast tRNA(Leu) (U*AA) is the only tRNA so far sequenced which has been shown to contain ncm5Um. The location of such a modified uridine at the first position of the anticodon restricts the decoding property to A of the leucine UUA codon.

Item Type: Article
Uncontrolled keywords: Trnaleu(U-Asterisk-Aa); Modified Nucleoside; Identification; Mass Spectrometry; Anticodon; Yeast
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 05 Sep 2009 14:42
Last Modified: 24 Apr 2014 08:02
Resource URI: https://kar.kent.ac.uk/id/eprint/22580 (The current URI for this page, for reference purposes)
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