Presence and Coding Properties of 2'-O-Methyl-5-Carbamoylmethyluridine (Ncm5um) in the Wobble Position of the Anticodon of Trnaleu (U-Asterisk-Aa) From Brewers-Yeast

Glasser, Anne-Lise, Eladlouni, Chakib, Keith, Gerard, Sochacka, Elzbieta, Malkiewicz, Andrzej, Santos, Manuel, Tuite, Mick F., Desgres, Jean (1992) Presence and Coding Properties of 2'-O-Methyl-5-Carbamoylmethyluridine (Ncm5um) in the Wobble Position of the Anticodon of Trnaleu (U-Asterisk-Aa) From Brewers-Yeast. FEBS Letters, 314 (3). pp. 381-385. ISSN 0014-5793. (doi:10.1016/0014-5793(92)81510-S) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:22580)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL: http://dx.doi.org/10.1016/0014-5793(92)81510-S

Abstract

The unknown modified nucleoside U* has been isolated by enzymatic and HPLC protocols from tRNA(Leu) (U*AA) recently discovered in brewer's yeast. The pure U* nucleoside has been characterized by electron impact mass spectroscopy, and comparison of its chromatographic and UV-absorption properties with those of appropriate synthetic compounds. The structure of U* was established as 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um). The yeast tRNA(Leu) (U*AA) is the only tRNA so far sequenced which has been shown to contain ncm5Um. The location of such a modified uridine at the first position of the anticodon restricts the decoding property to A of the leucine UUA codon.

Item Type:	Article
DOI/Identification number:	10.1016/0014-5793(92)81510-S
Uncontrolled keywords:	Trnaleu(U-Asterisk-Aa); Modified Nucleoside; Identification; Mass Spectrometry; Anticodon; Yeast
Subjects:	Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions:	Divisions > Division of Natural Sciences > Biosciences
Depositing User:	P. Ogbuji
Date Deposited:	05 Sep 2009 14:42 UTC
Last Modified:	05 Nov 2024 10:01 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/22580 (The current URI for this page, for reference purposes)

University of Kent Author Information

Santos, Manuel.

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CReDIT Contributor Roles:

Tuite, Mick F..

Creator's ORCID:	https://orcid.org/0000-0002-5214-540X
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