Woods, Adrian, Baines, Anthony J., Gull, Keith (1992) A high molecular mass phosphoprotein defined by a novel monoclonal antibody is closely associated with the intermicrotubule cross bridges in the Trypanosoma brucei cytoskeleton. Journal of Cell Science, 103 . pp. 665-675. ISSN 0021-9533. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:22286)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. |
Abstract
The main component of the cell body cytoskeleton of Trypanosoma brucei is the highly organised array of stable, subpellicular microtubules on the cytoplasmic face of the plasma membrane. Although several microtubule associated proteins (MAPs) have been shown to be associated with this array, the mechanisms by which individual microtubules interact with one another and with the membrane are still largely undetermined. In this study we have used the T. brucei cytoskeleton as a complex immunogen for the production of monoclonal antibodies to define novel cytoskeletal antigens. Screening by immunofluorescence enabled the selection of an antibody, WCB-1, which detects an antigen associated specifically with the subpellicular microtubules and not with the flagellum microtubules. The antigen (WCB210) was shown to have a relative molecular mass of 210 000 by western blotting. Immunogold studies showed the epitope to be located on the membrane-facing side of the subpellicular cage; it appears to be closely associated with the cross-bridges lying between the microtubules. Unlike many MAPs this protein was shown not to be heat stable and is predicted to be a roughly globular monomer. Even though WCB210 is a very minor component of the cytoskeleton it is heavily phosphorylated. It is possible that this protein is involved in regulation of the subpellicular microtubule crossbridges by interaction with other proteins.
Item Type: | Article |
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Uncontrolled keywords: | trypanosome; cytoskeleton; microtubule associated protein (map); wcb-1 |
Subjects: | Q Science |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | O.O. Odanye |
Date Deposited: | 27 Jul 2009 17:50 UTC |
Last Modified: | 05 Nov 2024 10:01 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/22286 (The current URI for this page, for reference purposes) |
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