Hovey, Alison, Bebbington, C.R, Jenkins, Nigel (1994) Control of Growth and Recombinant Protein-Synthesis by Heat-Shock - in a Mutant Mmmalian-Cell line. Biotechnology Letters, 16 (3). pp. 215-220. ISSN 0141-5492. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:20259)
| The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. |
Abstract
The effect of heat-shock on cell growth and the induction of recombinant protein synthesis by a temperature-sensitive (ts) Chinese hamster ovary (CHO) cell line was investigated. An optimal regime of successive 2 hour heat-shocks (42 degrees C) over 72 hours was found to simultaneously arrest cell growth and induce the synthesis of recombinant protein. The enhanced induction achieved from growth arrested cells may find application in the production of cytotoxic proteins.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QP Physiology (Living systems) > QP517 Biochemistry |
| Divisions: | Divisions > Division of Natural Sciences > Biosciences |
| Depositing User: | P. Ogbuji |
| Date Deposited: | 25 Jun 2009 13:19 UTC |
| Last Modified: | 05 Nov 2024 09:57 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/20259 (The current URI for this page, for reference purposes) |
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