Skip to main content
Kent Academic Repository

Mapping the binding site for matrix metalloproteinase on the N-terminal domain of the tissue inhibitor of metalloproteinases-2 by NMR chemical shift perturbation

Williamson, Richard A., Carr, Mark D., Frenkiel, Tom A., Feeney, James, Freedman, Robert B. (1997) Mapping the binding site for matrix metalloproteinase on the N-terminal domain of the tissue inhibitor of metalloproteinases-2 by NMR chemical shift perturbation. Biochemistry, 36 (45). pp. 13882-13889. ISSN 0006-2960. (doi:10.1021/bi9712091) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:18046)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1021/bi9712091

Abstract

Changes in the NMR chemical shift of backbone amide nuclei (H-1 and N-15) have been used to map the matrix metalloproteinase (MMP) binding site on the N-terminal domain of the tissue inhibitor of metalloproteinase-2 (N-TIMP-2). Amide chemical shift changes were measured on formation of a stable complex with the catalytic domain of stromelysin-l (N-MMP-3). Residues with significantly shifted amide signals mapped specifically to a broad site covering one face of the molecule. This site (the MMP binding site) consists primarily of residues 1-11, 27-41, 68-73, 87-90, and 97-104. The site overlaps with the OB-fold binding site seen in other proteins that share the same five-stranded beta-barrel topology. Sequence conservation data and recent site-directed mutagenesis studies are discussed in relation to the MMP binding site identified in this work.

Item Type: Article
DOI/Identification number: 10.1021/bi9712091
Additional information: 0006-2960 (Print) Journal Article Research Support, Non-U.S. Gov't
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: T.J. Sango
Date Deposited: 17 Apr 2009 11:57 UTC
Last Modified: 16 Nov 2021 09:56 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/18046 (The current URI for this page, for reference purposes)

University of Kent Author Information

Williamson, Richard A..

Creator's ORCID:
CReDIT Contributor Roles:

Freedman, Robert B..

Creator's ORCID:
CReDIT Contributor Roles:
  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.