Sohanpal, Baljinder K., Kulasekara, Hemantha Don, Bonnen, Alice, Blomfield, Ian C. (2001) Orientational control of fimE expression in Escherichia coli. Molecular Microbiology, 42 (2). pp. 483-494. ISSN 0950-382X. E-ISSN 1365-2958. (doi:10.1046/j.1365-2958.2001.02655.x) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:16)
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Abstract
Phase-variable expression of type 1 fimbriae is, in part, controlled by site-specific DNA inversion of the fim switch in Escherichia coli. Of the two fim recombinases (FimB and FimE) that catalyse the inversion reaction, FimE exhibits a strong bias for phase switching from the ON to the OFF orientation. The specificity associated with fimE is the result of two different mechanisms: (i) FimE exhibits a preference for the invertible element in the ON orientation as substrate for recombination; (ii) the invertible element in the OFF orientation acts in cis to inhibit recombinase activity (orientational control). We show here that the invertible element negatively regulates fimE, even though expression of a fimE-lacZYA transcriptional fusion is unaffected by orientational control. The fimE transcript extends into the invertible region and hence switch ON-specific and switch OFF-specific mRNA contain different sequences. Furthermore, we show that orientational control is suppressed by the insertion of a structured RNA (tRNA(Gly)) between fimE and the fim switch, indicating that the switch OFF-specific mRNA is inactivated by 3' to 5' degradation. Analysis of the fim switch reveals that it contains two inhibitory elements that exert orientational control independently.
Item Type: | Article |
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DOI/Identification number: | 10.1046/j.1365-2958.2001.02655.x |
Subjects: | Q Science |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | Susan Davies |
Date Deposited: | 19 Dec 2007 17:44 UTC |
Last Modified: | 16 Nov 2021 09:38 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/16 (The current URI for this page, for reference purposes) |
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