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tRNA and protein methylase complexes mediate zymocin toxicity in yeast

Studte, Patrick, Zink, Sabrina, Jablonowski, Daniel, Bar, Christian, von der Haar, Tobias, Tuite, Mick F., Schaffrath, Raffael (2008) tRNA and protein methylase complexes mediate zymocin toxicity in yeast. Molecular Microbiology, 69 (5). pp. 1266-1277. ISSN 0950-382X. (doi:10.1111/j.1365-2958.2008.06358.x) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:15241)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1111/j.1365-2958.2008.06358.x

Abstract

Modification of Saccharomyces cerevisiae tRNA anticodons at the wobble uridine (U34) position is required for tRNA cleavage by the zymocin tRNase killer toxin from Kluyveromyces lactis. Hence, U34 modification defects including lack of the U34 tRNA methyltransferase Trm9 protect against tRNA cleavage and zymocin. Using zymocin as a tool, we have identified toxin-resistant mutations in TRM9 that are likely to affect the U34 methylation reaction. Most strikingly, C-terminal truncations in Trm9 abolish interaction with Trm112, a protein shown to individually purify with Lys9 and two more methylases, Trm11 and Mtq2. Downregulation of a GAL1-TRM112 allele protects against zymocin whereas LYS9, TRM11 and MTQ2 are dosage suppressors of zymocin. Based on immune precipitation studies, the latter scenario correlates with competition for Trm112 and in excess, some of these Trm112 partners interfere with formation of the toxin-relevant Trm9.Trm112 complex. In contrast to trm11 Delta or lys9 Delta cells, trm112 Delta and mtq2 Delta null mutants are zymocin resistant. In line with the identified role that methylation of Sup45 by Mtq2 has for translation termination by the release factor dimer Sup45.Sup35, we observe that SUP45 overexpression and sup45 mutants suppress zymocin. Intriguingly, this suppression correlates with upregulated levels of tRNA species targeted by zymocin's tRNase activity.

Item Type: Article
DOI/Identification number: 10.1111/j.1365-2958.2008.06358.x
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QR Microbiology
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Louise Dorman
Date Deposited: 03 Sep 2009 13:44 UTC
Last Modified: 16 Nov 2021 09:53 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/15241 (The current URI for this page, for reference purposes)

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