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How do HYNIC-conjugated peptides bind technetium? Insights from LC-MS and stability studies (Abstract only)

Blower, Philip J., Surfraz, M. Bashir-Uddin, King, Robert C., Biagini, Stefano C. G., Mather, Stephen J. (2007) How do HYNIC-conjugated peptides bind technetium? Insights from LC-MS and stability studies (Abstract only). European Journal of Nuclear Medicine and Molecular Imaging, 34 (Sup 2). S148-S148. ISSN 1619-7070. (doi:10.1007/s00259-007-0547-6) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:13246)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1007/s00259-007-0547-6

Abstract

Hydrazinonicotinamide (HYNIC) is an established bifunctional complexing agent for technetium-99m (99mTc) but the structure of the technetium coordination sphere remains uncertain. To gain further insight into this, we have prepared conjugates of HYNIC and hydrazinobenzoic acid (HYBA) with a model peptide, and radiolabelled them with 99mTc using three well-established co-ligand systems: EDDA, tricine and tricine–nicotinic acid. The labelled peptides were studied by LC-MS and by subjecting them to serum stability and protein binding assays. For each co-ligand system, HYNIC conjugates formed fewer and more stable labelled species than the corresponding HYBA conjugates. LC-MS analysis showed that all conjugates contained one hydrazine moiety bound to Tc, that binding of Tc to HYNIC–peptide and co-ligand occurs with displacement of 5H+ indicating a Tc formal oxidation state of +5, and that the Tc has no oxo- or halide ligands. LC-MS also shows that complexes formed with the HYNIC conjugate contain fewer coordinating co-ligand molecules than the HYBA conjugate indicating that HYNIC is able to more effectively satisfy the coordination requirement of technetium, perhaps by binding in chelating mode.

Item Type: Article
DOI/Identification number: 10.1007/s00259-007-0547-6
Additional information: Abstracts of the Annual Congress of the EANM 2007, Copenhagen, Denmark
Subjects: Q Science
Q Science > QD Chemistry
R Medicine
Divisions: Divisions > Division of Natural Sciences > Physics and Astronomy
Depositing User: Stefano Biagini
Date Deposited: 07 Sep 2009 12:12 UTC
Last Modified: 16 Nov 2021 09:51 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/13246 (The current URI for this page, for reference purposes)

University of Kent Author Information

Blower, Philip J..

Creator's ORCID:
CReDIT Contributor Roles:

Surfraz, M. Bashir-Uddin.

Creator's ORCID:
CReDIT Contributor Roles:

Biagini, Stefano C. G..

Creator's ORCID: https://orcid.org/0000-0002-4713-5127
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