Effectiveness evaluation of microbiological preparations for preserving ensiled plant feeds in a model experiment using microbiomic and bioinformatic tools

Filippova, Valentina A., Ilina, Larisa A., Yildirim, Elena A., Dubrovin, Andrei V., Ponomareva, Ekaterina S., Dubrovina, Alisa S., Klyuchnikova, Irina A., Zaikin, Vasiliy A., Malakhov, Ivan G., Laptev, Georgi Yu., and others. (2026) Effectiveness evaluation of microbiological preparations for preserving ensiled plant feeds in a model experiment using microbiomic and bioinformatic tools. In: Agriculture Digitalization and Organic Production: Proceedings of the Fifth International Conference on Agriculture Digitalization and Organic Production (ADOP 2025), Volume 2, Barnaul, Russia, 3–6 June 2025. Smart Innovation, Systems and Technologies Springer, Cham, Switzerland ISBN 978-3-032-17406-2. E-ISBN 978-3-032-17407-9. (doi:10.1007/978-3-032-17407-9_29) (KAR id:115818)

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Official URL: https://doi.org/10.1007/978-3-032-17407-9_29
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Abstract

Ensiling is the main method of preparing bulk forages for cattle in the conditions of the risk farming zone. This zone includes St. Petersburg and the Leningrad Oblast due to their geographical location and high humidity. To improve the efficiency of enzymatic processes during ensiling, various biopreparations of lactic acid bacteria that consist of one or more strains are used. However, the biotechnological potential of lactic acid bacteria involved in silage fermentation remains insufficiently studied. Thus, the selection of microorganisms for use in silages should always be carried out with all rigor and meet certain criteria. The aim of this study using metagenomic next-generation sequencing (NGS) and bioinformatics was to assess the efficiency of applying monocultures of lactic acid bacteria strains (Lactobacillus plantarum 50 and Enterococcus faecium 46). We further evaluated combining these strains for the ensiling process in a model laboratory experiment. As a result, it was shown that the greatest stability of microbiome and a high proportion of lactobacilli in the ensiled feeds, the best pH levels and silage quality were achieved using a combination of strains (L. plantarum 50 + E. faecium 46).

Item Type:	Conference proceeding
DOI/Identification number:	10.1007/978-3-032-17407-9_29
Projects:	Development of a comprehensive biotechnological approach for biological protection of cattle and livestock products from pathogenic bacteria and their toxins
Uncontrolled keywords:	Biopreparations, strains, bacteria, plant feed, ensiled feeds, microbiome, metagenomic next-generation sequencing (NGS), endotoxin genes, bioinformatics
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology S Agriculture > SF Animal culture
Institutional Unit:	Schools > School of Natural Sciences Schools > School of Natural Sciences > Biosciences
Former Institutional Unit:	There are no former institutional units.
Depositing User:	Mike Romanov
Date Deposited:	31 Jul 2026 11:03 UTC
Last Modified:	27 Aug 2026 11:51 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/115818 (The current URI for this page, for reference purposes)

University of Kent Author Information

Griffin, Darren K..

Creator's ORCID:	https://orcid.org/0000-0001-7595-3226
CReDIT Contributor Roles:

Romanov, Michael N.

Creator's ORCID:	https://orcid.org/0000-0003-3584-4644
CReDIT Contributor Roles:

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