Marotta, Maria Giovanna, Neto, Martin Mayora, Daly, Janet, Maina, Meshach, van Diemen, Pauline, Everett, Helen, Lucente, Maria Stella, Camero, Michele, Montomoli, Emanuele, Trombetta, Claudia Maria, and others. (2025) Development and optimisation of influenza C and influenza D pseudotyped viruses. Journal of Virological Methods, 339 . Article Number 115243. ISSN 0166-0934. (doi:10.1016/j.jviromet.2025.115243) (KAR id:111186)
|
PDF
Publisher pdf
Language: English 
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
|
|
|
Download this file (PDF/2MB) |
Preview |
| Request a format suitable for use with assistive technology e.g. a screenreader | |
| Official URL: https://doi.org/10.1016/j.jviromet.2025.115243 |
|
Abstract
To facilitate the study of influenza C (ICV) and influenza D (IDV) viruses, we generated lentiviral pseudotyped viruses (PVs) expressing the hemagglutinin-esterase fusion (HEF) glycoprotein from ICV (C/Minnesota/33/2015) and IDV (D/Swine/Italy/199724-3/2015, D/Bovine/France/5920/2014, and D/Bovine/Ibaraki/7768/2016). The production of these PVs was optimised using different amount of human airway trypsin-like (HAT) protease to enhance HEF maturation, and the transduction efficiency was evaluated in multiple cell lines. Using these PVs, we established a pseudovirus-based microneutralisation (pMN) assay to measure neutralising antibody responses and adapted an esterase activity assay to evaluate PV. Specific antisera neutralised PVs but failed to inhibit esterase activity. These findings confirm that ICV and IDV PVs provide a scalable, sensitive, and safe tool for antiviral screening, and sero-epidemiological research.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.1016/j.jviromet.2025.115243 |
| Uncontrolled keywords: | hemagglutinin esterase fusion; immunity; influenza D virus; pseudotyped virus; influenza C virus |
| Subjects: | R Medicine |
| Institutional Unit: | Schools > Medway School of Pharmacy |
| Former Institutional Unit: |
There are no former institutional units.
|
| Funders: | European Union (https://ror.org/019w4f821) |
| SWORD Depositor: | JISC Publications Router |
| Depositing User: | JISC Publications Router |
| Date Deposited: | 18 Sep 2025 08:35 UTC |
| Last Modified: | 20 Sep 2025 03:22 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/111186 (The current URI for this page, for reference purposes) |
University of Kent Author Information
Neto, Martin Mayora.
| Creator's ORCID: | |
|---|---|
| CReDIT Contributor Roles: | Writing - review and editing, Investigation |
da Costa, Kelly.
| Creator's ORCID: |
 https://orcid.org/0000-0001-6352-3843
|
|---|---|
| CReDIT Contributor Roles: | Conceptualisation, Investigation, Supervision, Data curation, Writing - review and editing |
Temperton, Nigel J..
| Creator's ORCID: |
https://orcid.org/0000-0002-7978-3815
|
|---|---|
| CReDIT Contributor Roles: | Conceptualisation, Resources, Funding acquisition, Supervision, Writing - review and editing |
- Link to SensusAccess
- Export to:
- RefWorks
- EPrints3 XML
- BibTeX
- CSV
- Depositors only (login required):
Altmetric
Altmetric
