X-ray structure of a putative reaction intermediate of 5-aminolaevulinic acid dehydratase

Erskine, Peter T., Coates, Leighton, Butler, Danica, Youell, James H., Brindley, Amanda A., Wood, Steve P., Warren, Martin J., Shoolingin-Jordan, Peter M., Cooper, Jonathan B. (2003) X-ray structure of a putative reaction intermediate of 5-aminolaevulinic acid dehydratase. Biochemical Journal, 373 (Pt 3). pp. 733-8. ISSN 0264-6021. (doi:10.1042/BJ20030513) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:11052)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL: http://dx.doi.org/10.1042/BJ20030513

Abstract

The X-ray structure of yeast 5-aminolaevulinic acid dehydratase, in which the catalytic site of the enzyme is complexed with a putative cyclic intermediate composed of both substrate moieties, has been solved at 0.16 nm (1.6 A) resolution. The cyclic intermediate is bound covalently to Lys(263) with the amino group of the aminomethyl side chain ligated to the active-site zinc ion in a position normally occupied by a catalytic hydroxide ion. The cyclic intermediate is catalytically competent, as shown by its turnover in the presence of added substrate to form porphobilinogen. The findings, combined with those of previous studies, are consistent with a catalytic mechanism in which the C-C bond linking both substrates in the intermediate is formed before the C-N bond.

Item Type:	Article
DOI/Identification number:	10.1042/BJ20030513
Uncontrolled keywords:	Crystallography, X-Ray Models, Molecular Porphobilinogen Synthase/*chemistry/metabolism Protein Structure, Tertiary Recombinant Proteins/chemistry/metabolism Saccharomyces cerevisiae/enzymology
Subjects:	Q Science
Divisions:	Divisions > Division of Natural Sciences > Biosciences
Depositing User:	Martin Warren
Date Deposited:	09 Oct 2009 08:04 UTC
Last Modified:	05 Nov 2024 09:44 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/11052 (The current URI for this page, for reference purposes)

University of Kent Author Information

Warren, Martin J..

Creator's ORCID:	https://orcid.org/0000-0002-6028-6456
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