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Effects of glyphosate, antibiotics and an anticoccidial drug on pancreatic gene expression and blood physiology in broilers

Laptev, Georgi Yu., Tiurina, Darya G., Yildirim, Elena A., Gorfunkel, Elena P., Ilina, Larisa A., Filippova, Valentina A., Dubrovin, Andrei V., Dubrovina, Alisa S., Brazhnik, Evgeni A., Novikova, Natalia I., and others. (2025) Effects of glyphosate, antibiotics and an anticoccidial drug on pancreatic gene expression and blood physiology in broilers. Journal of Zhejiang University-SCIENCE B, 26 (2). pp. 185-199. ISSN 1673-1581. E-ISSN 1862-1783. (doi:10.1631/jzus.B2300767) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:108905)

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Official URL:
https://doi.org/10.1631/jzus.B2300767

Abstract

Drugs and pesticide residues in broiler feed can compromise the therapeutic and production benefits of antibiotic (ANT) application and affect gene expression. In this study, we analyzed the expression of 13 key pancreatic genes and blood physiology parameters after administering one maximum residue limit of herbicide glyphosate (GLY), two ANTs, and one anticoccidial drug (AD). A total of 260 Ross 308 broilers aged 1‍–‍40 d were divided into the following four groups of 65 birds each: control group, which was fed the main diet (MD), and three experimental groups, which were fed MD supplemented with GLY, GLY+ANTs (enrofloxacin and colistin methanesulfonate), and GLY+AD (ammonium maduramicin), respectively. The results showed that the addition of GLY, GLY+ANTs, and GLY+AD caused significant changes in the expression of several genes of physiological and economic importance. In particular, genes related to inflammation and apoptosis (interleukin 6 (IL6), prostaglandin-endoperoxide synthase 2 (PTGS2), and caspase 6 (CASP6)) were downregulated by up to 99.1%, and those related to antioxidant protection (catalase (CAT), superoxide dismutase 1 (SOD1) and peroxiredoxin 6 (PRDX6)) by up to 98.6%, compared to controls. There was also a significant decline in the values of immunological characteristics in the blood serum observed in the experimental groups, and certain changes in gene expression were concordant with changes in the functioning of the pancreas and blood. The changes revealed in gene expression and blood indices in response to GLY, ANTs, and AD provide insights into the possible mechanisms of action of these agents at the molecular level. Specifically, these changes may be indicative of physiological mechanisms to overcome the negative effects of GLY, GLY+ANTs, and GLY+AD in broilers.

Item Type: Article
DOI/Identification number: 10.1631/jzus.B2300767
Projects: Investigation of the Toxic Effect of Glyphosates on the Functional State of the Bird Intestinal Microbial Community, Their Growth and Development, and the Development of a Biological Product Based on the Glyphosate Degrading Strain
Uncontrolled keywords: glyphosate, antibiotic, anticoccidial drug, pancreas, broiler, gene expression, blood parameter
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QP Physiology (Living systems)
Q Science > QP Physiology (Living systems) > QP517 Biochemistry
S Agriculture > SF Animal culture
Divisions: Divisions > Division of Natural Sciences > Centre for Interdisciplinary Studies of Reproduction
Divisions > Division of Natural Sciences > Biosciences
Depositing User: Mike Romanov
Date Deposited: 27 Feb 2025 13:02 UTC
Last Modified: 05 Mar 2025 10:47 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/108905 (The current URI for this page, for reference purposes)

University of Kent Author Information

Griffin, Darren K..

Creator's ORCID: https://orcid.org/0000-0001-7595-3226
CReDIT Contributor Roles: Supervision, Writing - review and editing

Romanov, Michael N.

Creator's ORCID: https://orcid.org/0000-0003-3584-4644
CReDIT Contributor Roles: Visualisation, Writing - original draft, Writing - review and editing, Data curation
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