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An orthogonal approach for analysis of underivatized steroid hormones using ultrahigh performance supercritical fluid chromatography-mass spectrometry (UHPSFC-MS).

Devo, Perry, Cretu, Victoria, Radhakrishnan, Harsha, Hamilton-Pink, Darren, Boussios, Stergios, Ovsepian, Saak V. (2024) An orthogonal approach for analysis of underivatized steroid hormones using ultrahigh performance supercritical fluid chromatography-mass spectrometry (UHPSFC-MS). Journal of neural transmission (Vienna, Austria : 1996), . ISSN 0300-9564. E-ISSN 1435-1463. (doi:10.1007/s00702-024-02862-3) (KAR id:107966)

Abstract

The crucial role of steroid hormones in health and diseases merits their high-throughput, accurate and affordable measurements in biological specimens. Despite advances in analytical methods, sensing and quantifying steroid hormones remains challenging. Immunoassays offer excellent sensitivity but are inherently labour-intensive, costly, and prone to false positives. Mass spectrometry (MS) has been increasingly utilised, with the main hurdle being the isobaric tendencies of similar analytes, which complicates their separation and accurate quantification. This study compares ultrahigh-performance supercritical fluid chromatography separation (UHPSFC) and ultra-high-performance liquid chromatography (UHPLC) for MS detection. It optimises the column chemistry, temperature, and pressure to provide an operational protocol for the resolution and quantification of analytes. It presents the systematic characterisation of UHPSFC-MS performance by investigating spiked blood samples using Solid-Phase Extraction (SPE) and describes the matrix effects associated with MS measurements. Although both separation methods showed adequate resolution, specificity, and retention time, UHPSFC-MS was superior for five out of seven columns tested. With added high-throughput capacities, UHPSFC-MS, thus, offers an optimal solution for the analysis of steroid hormones for research, medical chemistry, and clinical diagnostics.

Item Type: Article
DOI/Identification number: 10.1007/s00702-024-02862-3
Uncontrolled keywords: Supercritical fluid, Biomarkers, Steroid hormones, UHPSFC-MS, UHPLC-MS, Solid phase extraction
Subjects: R Medicine
Divisions: Divisions > Division of Natural Sciences > Kent and Medway Medical School
Funders: University of Greenwich (https://ror.org/00bmj0a71)
SWORD Depositor: JISC Publications Router
Depositing User: JISC Publications Router
Date Deposited: 28 Nov 2024 14:19 UTC
Last Modified: 29 Nov 2024 10:53 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/107966 (The current URI for this page, for reference purposes)

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