Nteroli, Gianni, Dasa, Manoj K, Messa, Giulia, Koutsikou, Stella, Bondu, Magalie M., Moselund, Peter M., Markos, Christos, Bang, Ole, Podoleanu, Adrian G. H., Bradu, Adrian and others. (2024) Development of a combined multi-spectral optoacoustic microscopy and optical coherence tomography imaging instrument for mapping multiple chromophores in biological tissues. In: Proceedings Volume 13006 SPIE Photonics Europe. 13006. p. 42. SPIE (doi:10.1117/12.3017439) (KAR id:106796)
PDF
Author's Accepted Manuscript
Language: English
This work is licensed under a Creative Commons Attribution 4.0 International License.
|
|
Download this file (PDF/632kB) |
Preview |
Request a format suitable for use with assistive technology e.g. a screenreader | |
Official URL: https://doi.org/10.1117/12.3017439 |
Abstract
This report presents a state-of-the-art multimodality imaging device that combines multi-spectral optoacoustic microscopy (OAM) and optical coherence tomography (OCT) to chart absorbers in live tadpoles (Xenopus laevis) accurately. The OAM channel captures maps of five internal contrast agents: melanin, haemoglobin, collagen, glucose, and lipids. A novel
method was developed to achieve this by assuming that each voxel in the 3D-OAM image exhibits a single chromophore
contributing to the optoacoustic signal. The device is powered by a single optical source (SuperK Compact, NKT
Photonics) that operates across an ultra-wide spectral range of 450 to 2400 nm. The set-up was optimized by minimizing optical aberrations and attenuation on optical components to stimulate the sample effectively. Using optical pulses of 2 ns duration and a repetition rate of 20 kHz, the device imaged tadpoles in their embryonic stage at multiple wavelengths, using narrow spectral windows of 25 nm bandwidth within the broad spectrum of the supercontinuum source at a time.
In addition, an ultra-high-resolution OCT imaging channel operating at 1300 nm (spectral bandwidth 180 nm) was created and incorporated into the device. The OCT channel, also powered by a commercial supercontinuum source (SuperK EXTREME EXR9, NKT Photonics), was used for guidance purposes and to help determine the location of the chromophores.
Item Type: | Conference or workshop item (Keynote) |
---|---|
DOI/Identification number: | 10.1117/12.3017439 |
Uncontrolled keywords: | optoacoustic microscopy; optical coherence tomography; multimodality imaging; tadpoles |
Subjects: | Q Science > QC Physics > QC355 Optics |
Divisions: | Divisions > Division of Natural Sciences > Physics and Astronomy |
Funders: |
Academy of Medical Sciences (https://ror.org/00c489v88)
Physiological Society (https://ror.org/003pxap67) |
Depositing User: | Adrian Bradu |
Date Deposited: | 05 Aug 2024 09:11 UTC |
Last Modified: | 07 Aug 2024 08:48 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/106796 (The current URI for this page, for reference purposes) |
- Link to SensusAccess
- Export to:
- RefWorks
- EPrints3 XML
- BibTeX
- CSV
- Depositors only (login required):