Nteroli, Gianni (2024) Developing opto-acoustic microscopy instruments for biomedical imaging applications. Doctor of Philosophy (PhD) thesis, University of Kent,. (doi:10.22024/UniKent/01.02.105951) (KAR id:105951)
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Official URL: https://doi.org/10.22024/UniKent/01.02.105951 |
Abstract
Opto-acoustic Microscopy is an emerging technique for cross-sectional imaging that provides structural and functional volumetric information with micrometer resolution. This allows for non-invasive detection of endogenous contrast agents and chromophores without using ionizing radiation. The objective of this thesis is to investigate the potential of opto-acoustic microscopy combined with optical coherence tomography in developing advanced diagnostic tools for biomedical applications, in particular for cancer diagnosis. To achieve this objective, the thesis focuses on in-vivo multi-spectral optoacoustic microscopy imaging of multiple endogenous contrast agents in Xenopus laevis. The study used a high-resolution opto-acoustic microscopy instrument capable of multi-pectral imaging covering two octaves of the spectrum, and a novel technique to distinguish between different chromophores in the sample. An optical coherence tomography instrument was integrated in the opto-acoustic microscopy system to guide imaging and provide reliable structural information. Additionally, visible light optical coherence tomography system was developed as an ultra-high resolution alternative. Prior to this study, mapping of lipids in Xenopus laevis was achieved using an in-house all-fibre supercontinuum optical source developed in DTU, Denmark operating in the extended near-infrared region. Both opto-acoustic microscopy and optical coherence tomography instruments are capable of acquiring cross-sectional and volumetric images in real-time. Finally, a high-resolution opto-acoustic microscopy set-up to explore the impact of picosecond pulse duration excitation on the axial resolution of the imaging system. The study compared a picosecond pulse duration laser-based optoacoustic microscopy instrument to a nanosecond laser-based one in terms of axial resolution and obtained unprecedented in-vivo images of the brain in Xenopus laevis tadpoles.
Item Type: | Thesis (Doctor of Philosophy (PhD)) |
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Thesis advisor: | Bradu, Adrian |
DOI/Identification number: | 10.22024/UniKent/01.02.105951 |
Uncontrolled keywords: | optoacoustic microscopy, optical coherence tomography, OCT, PA, biomedical, imaging, physics. |
Subjects: | Q Science > QC Physics |
Divisions: | Divisions > Division of Natural Sciences > Physics and Astronomy |
SWORD Depositor: | System Moodle |
Depositing User: | System Moodle |
Date Deposited: | 13 May 2024 09:10 UTC |
Last Modified: | 16 May 2024 14:11 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/105951 (The current URI for this page, for reference purposes) |
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