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Complex master-slave enhanced optical coherence microscopy

Camard, Julien, Marques, Manuel J., Canedo-Ribeiro, Carla, Silvestri, M., Ellis, Peter J.I., Robinson, Gary K., Chavez-Badiola, Alejandro, Griffin, Darren K., Bradu, Adrian, Podoleanu, Adrian G.H. and others. (2024) Complex master-slave enhanced optical coherence microscopy. Optics Continuum, 3 (3). pp. 324-337. E-ISSN 2770-0208. (doi:10.1364/OPTCON.518366) (KAR id:105139)

Abstract

We present an instrument designed to facilitate localization and high-resolution, optical coherence microscopy (OCM) imaging of small biological samples immersed in a medium several orders of magnitude greater in volume. A modified turret-equipped microscope stand was inserted into the sample arm of a spectral domain optical coherence microscopy (SD-OCM) system. The instrument enabled swift change of imaging objectives through the incorporation of complex master-slave interferometry (CMSI), providing tolerance to dispersion for any objective through the acquisition of a few (≥2) calibration spectra. We demonstrate the instrument’s ability to localize and image samples by providing examples of its application to optical phantoms and to a porcine oocyte immersed in a biological culture medium.

Item Type: Article
DOI/Identification number: 10.1364/OPTCON.518366
Subjects: Q Science > QC Physics > QC355 Optics
Divisions: Divisions > Division of Natural Sciences > Physics and Astronomy
Funders: Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982)
Depositing User: Julien Camard
Date Deposited: 28 Feb 2024 10:55 UTC
Last Modified: 29 Feb 2024 10:24 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/105139 (The current URI for this page, for reference purposes)

University of Kent Author Information

Camard, Julien.

Creator's ORCID: https://orcid.org/0009-0001-9024-6914
CReDIT Contributor Roles:

Marques, Manuel J..

Creator's ORCID: https://orcid.org/0000-0002-7509-0704
CReDIT Contributor Roles:

Canedo-Ribeiro, Carla.

Creator's ORCID: https://orcid.org/0000-0003-1575-7420
CReDIT Contributor Roles:

Silvestri, M..

Creator's ORCID: https://orcid.org/0000-0002-8351-6285
CReDIT Contributor Roles:

Ellis, Peter J.I..

Creator's ORCID: https://orcid.org/0000-0001-9709-7934
CReDIT Contributor Roles:

Robinson, Gary K..

Creator's ORCID: https://orcid.org/0000-0003-2660-7778
CReDIT Contributor Roles:

Chavez-Badiola, Alejandro.

Creator's ORCID: https://orcid.org/0000-0002-7977-3174
CReDIT Contributor Roles:

Griffin, Darren K..

Creator's ORCID: https://orcid.org/0000-0001-7595-3226
CReDIT Contributor Roles:

Bradu, Adrian.

Creator's ORCID: https://orcid.org/0000-0002-6890-1599
CReDIT Contributor Roles:

Podoleanu, Adrian G.H..

Creator's ORCID: https://orcid.org/0000-0002-4899-9656
CReDIT Contributor Roles:
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