Camard, Julien, Marques, Manuel J., Canedo-Ribeiro, Carla, Silvestri, M., Ellis, Peter J.I., Robinson, Gary K., Chavez-Badiola, Alejandro, Griffin, Darren K., Bradu, Adrian, Podoleanu, Adrian G.H. and others. (2024) Complex master-slave enhanced optical coherence microscopy. Optics Continuum, 3 (3). pp. 324-337. E-ISSN 2770-0208. (doi:10.1364/OPTCON.518366) (KAR id:105139)
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Official URL: https://doi.org/10.1364/OPTCON.518366 |
Abstract
We present an instrument designed to facilitate localization and high-resolution, optical coherence microscopy (OCM) imaging of small biological samples immersed in a medium several orders of magnitude greater in volume. A modified turret-equipped microscope stand was inserted into the sample arm of a spectral domain optical coherence microscopy (SD-OCM) system. The instrument enabled swift change of imaging objectives through the incorporation of complex master-slave interferometry (CMSI), providing tolerance to dispersion for any objective through the acquisition of a few (≥2) calibration spectra. We demonstrate the instrument’s ability to localize and image samples by providing examples of its application to optical phantoms and to a porcine oocyte immersed in a biological culture medium.
Item Type: | Article |
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DOI/Identification number: | 10.1364/OPTCON.518366 |
Subjects: | Q Science > QC Physics > QC355 Optics |
Divisions: | Divisions > Division of Natural Sciences > Physics and Astronomy |
Funders: | Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982) |
Depositing User: | Julien Camard |
Date Deposited: | 28 Feb 2024 10:55 UTC |
Last Modified: | 29 Feb 2024 10:24 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/105139 (The current URI for this page, for reference purposes) |
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