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Differential temporal release and lipoprotein loading in B. thetaiotaomicron bacterial extracellular vesicles

Juodeikis, Rokas, Martins, Carlo, Saalbach, Gerhard, Richardson, Jake, Koev, Trey, Baker, Dave J., Defernez, Marianne, Warren, Martin J., Carding, Simon R. (2024) Differential temporal release and lipoprotein loading in B. thetaiotaomicron bacterial extracellular vesicles. Journal of Extracellular Vesicles, 13 (1). Article Number 12406. ISSN 2001-3078. (doi:10.1002/jev2.12406) (KAR id:104673)

Abstract

Bacterial extracellular vesicles (BEVs) contribute to stress responses, quorum sensing, biofilm formation and interspecies and interkingdom communication. However, the factors that regulate their release and heterogeneity are not well understood. We set out to investigate these factors in the common gut commensal Bacteroides thetaiotaomicron by studying BEV release throughout their growth cycle. Utilising a range of methods, we demonstrate that vesicles released at different stages of growth have significantly different composition, with early vesicles enriched in specifically released outer membrane vesicles (OMVs) containing a larger proportion of lipoproteins, while late phase BEVs primarily contain lytic vesicles with enrichment of cytoplasmic proteins. Furthermore, we demonstrate that lipoproteins containing a negatively charged signal peptide are preferentially incorporated in OMVs. We use this observation to predict all Bacteroides thetaiotaomicron OMV enriched lipoproteins and analyse their function. Overall, our findings highlight the need to understand media composition and BEV release dynamics prior to functional characterisation and define the theoretical functional capacity of Bacteroides thetaiotaomicron OMVs.

Item Type: Article
DOI/Identification number: 10.1002/jev2.12406
Additional information: For the purpose of open access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission.
Uncontrolled keywords: proteomics, OMV, BEV, outer membrane vesicles, bacterial extracellular vesicles, Bacteroides
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982)
Royal Society (https://ror.org/03wnrjx87)
SWORD Depositor: JISC Publications Router
Depositing User: JISC Publications Router
Date Deposited: 19 Jan 2024 15:31 UTC
Last Modified: 22 Jan 2024 11:17 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/104673 (The current URI for this page, for reference purposes)

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