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Correlation between pseudotyped virus and authentic virus neutralisation assays, a systematic review and meta-analysis of the literature

Cantoni, Diego, Wilkie, Craig, Bentley, Emma, Mayora-Neto, Martin, Wright, Edward, Scott, Simon D., Ray, Surajit, Castillo-Olivares, Javier, Heeney, Jonathan L., Mattiuzzo, Giada, and others. (2023) Correlation between pseudotyped virus and authentic virus neutralisation assays, a systematic review and meta-analysis of the literature. Frontiers in Immunology, 14 . ISSN 1664-3224. (doi:10.3389/fimmu.2023.1184362) (KAR id:102589)


Background: The virus neutralization assay is a principal method to assess the efficacy of antibodies in blocking viral entry. Due to biosafety handling requirements of viruses classified as hazard group 3 or 4, pseudotyped viruses can be used as a safer alternative. However, it is often queried how well the results derived from pseudotyped viruses correlate with authentic virus. This systematic review and meta-analysis was designed to comprehensively evaluate the correlation between the two assays.

Methods: Using PubMed and Google Scholar, reports that incorporated neutralisation assays with both pseudotyped virus, authentic virus, and the application of a mathematical formula to assess the relationship between the results, were selected for review. Our searches identified 67 reports, of which 22 underwent a three-level meta-analysis.

Results: The three-level meta-analysis revealed a high level of correlation between pseudotyped viruses and authentic viruses when used in an neutralisation assay. Reports that were not included in the meta-analysis also showed a high degree of correlation, with the exception of lentiviral-based pseudotyped Ebola viruses.

Conclusion: Pseudotyped viruses identified in this report can be used as a surrogate for authentic virus, though care must be taken in considering which pseudotype core to use when generating new uncharacterised pseudotyped viruses.

Item Type: Article
DOI/Identification number: 10.3389/fimmu.2023.1184362
Additional information: For the purpose of open access, the author(s) has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising.
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Divisions > Division of Natural Sciences > Medway School of Pharmacy
Funders: Medical Research Council (
Depositing User: Nigel Temperton
Date Deposited: 29 Aug 2023 12:11 UTC
Last Modified: 27 Feb 2024 11:55 UTC
Resource URI: (The current URI for this page, for reference purposes)

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