Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38

Maloney, Jenny G, Molokin, Aleksey, Seguí, Raimundo, Maravilla, Pablo, Martínez-Hernández, Fernando, Villalobos, Guiehdani, Tsaousis, Anastasios D., Gentekaki, Eleni, Muñoz-Antolí, Carla, Klisiowicz, Debora R, and others. (2022) Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38. Microorganisms, 11 (1). Article Number 46. ISSN 2076-2607. (doi:10.3390/microorganisms11010046) (KAR id:99892)

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Official URL: https://doi.org/10.3390/microorganisms11010046

Abstract

Three recent studies of epidemiology in mammalian hosts identified four novel sequences that appeared to share as the most similar sequence. However, full-length rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were performed to confirm the validity of each of these new subtypes. We propose that the novel sequences described in this study should be assigned the subtype designations ST35-ST38. The full-length reference sequences of ST35-ST38 will assist in accurate sequence descriptions in future studies of epidemiology and subtype diversity.

Item Type:	Article
DOI/Identification number:	10.3390/microorganisms11010046
Additional information:	For the purpose of open access, the author(s) has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising.
Uncontrolled keywords:	Blastocystis; B. lapemi; subtype; Oxford Nanopore MinION; ssu rRNA
Subjects:	Q Science Q Science > QH Natural history > QH301 Biology
Divisions:	Divisions > Division of Natural Sciences > Biosciences
Funders:	Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982)
SWORD Depositor:	JISC Publications Router
Depositing User:	JISC Publications Router
Date Deposited:	08 Feb 2023 15:26 UTC
Last Modified:	20 Nov 2023 15:40 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/99892 (The current URI for this page, for reference purposes)
Maloney, Jenny G:	https://orcid.org/0000-0002-6405-883X
Tsaousis, Anastasios D.:	https://orcid.org/0000-0002-5424-1905
Muñoz-Antolí, Carla:	https://orcid.org/0000-0003-1778-4297
Klisiowicz, Debora R:	https://orcid.org/0000-0002-7699-0763
Toledo, Rafael:	https://orcid.org/0000-0003-2651-9794
Esteban, J Guillermo:	https://orcid.org/0000-0001-7265-8459
Dashti, Alejandro:	https://orcid.org/0000-0001-8707-5731
Calero-Bernal, Rafael:	https://orcid.org/0000-0003-2323-0135
González-Barrio, David:	https://orcid.org/0000-0001-5083-8854
Carmena, David:	https://orcid.org/0000-0002-4015-8553
Santín, Mónica:	https://orcid.org/0000-0002-1386-6255

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