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A conserved karyotype? Chromosomal rearrangements in Charadrius collaris detected by BAC-FISH

Ferreira, Paulo Victor de Moraes, Ribas, Talita Fernanda Augusto, Griffin, Darren K., Correa, Luyann André Rodrigues, Pinheiro, Melquizedec Luiz Silva, Kiazim, Lucas Gem, O’Connor, Rebecca E., Nagamachi, Cleusa Yoshiko, Pieczarka, Julio Cesar (2023) A conserved karyotype? Chromosomal rearrangements in Charadrius collaris detected by BAC-FISH. PLOS ONE, 18 (1). Article Number e0280164. ISSN 1932-6203. (doi:10.1371/journal.pone.0280164) (KAR id:99539)


Charadriidae comprise 142 valid species and the most recent checklist for the occurrence of this family in Brazil describes 11 species. There are few chromosomal studies in Charadriidae, most of them using a conventional approach. In Charadrius, only five species had their karyotypes described by classical cytogenetics, of which four have 2n = 76 (C. hiaticula, C. dubius, C. vociferou and C. collaris) and one 2n = 78 (C. alexandrinus alexandrinus). Among these species, only Charadrius collaris had the karyotype studied by chromosome painting, which allowed the identification of chromosomal homeologies with the karyotypes of Gallus gallus (GGA) and Burhinus oedicnemus (BOE). According to the literature, studies performed with BAC-FISH using probes from Gallus gallus and Taeniopygia guttata (TGU) libraries have shown interactions between macro and microchromosomes and micro inversions in chromosomes previously considered conserved. Other studies have shown the fusion of several microchromosomes, forming new macrochromosomes, leading to a decrease in the 2n of some species. The present study aims to deepen the chromosomal information in Charadrius collaris through the application of BAC-FISH with probes from the GGA and TGU libraries, in order to investigate possible rearrangements within the apparently conserved karyotype of this species, and thus better clarify the evolutionary history of the species. Charadrius collaris presented 2n = 76 and fundamental number (FN) equal to 94. Comparative mapping of BAC probes from GGA and TGU in Charadrius collaris revealed hybridization signals from 26 macrochromosome probes. Probes from microchromosomes 9 to 28 of GGA were also used and revealed 31 hybridization signals. The karyotype is well conserved, but it contains a paracentric and a pericentric inversion on the CCO1 chromosome, a paracentric and a pericentric inversion on the CCO4 and the separation of GGA4 into CCO4 and CCO8, demonstrating that the BAC-FISH approach allows for greater data resolution. More studies are needed to improve the understanding of chromosomal evolution within the order Charadriiformes and thus clarify whether these characteristics demonstrated here are specific traits for Charadrius collaris or if other species share these characteristics.

Item Type: Article
DOI/Identification number: 10.1371/journal.pone.0280164
Additional information: For the purpose of open access, the author(s) has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising.
Uncontrolled keywords: animals, chickens, songbirds, chromosome painting, karyotyping, evolution, molecular, charadriiformes, karyotype
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: Biotechnology and Biological Sciences Research Council (
Depositing User: Darren Griffin
Date Deposited: 16 Jan 2023 11:47 UTC
Last Modified: 20 Nov 2023 15:38 UTC
Resource URI: (The current URI for this page, for reference purposes)

University of Kent Author Information

Griffin, Darren K..

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Kiazim, Lucas Gem.

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O’Connor, Rebecca E..

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