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Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species

de Souza, Marcelo Santos, Barcellos, Suziane Alves, Dos Santos, Michelly da Silva, Gunski, Ricardo José, Garnero, Analía Del Valle, de Oliveira, Edivaldo Herculano Corrêa, O'Connor, Rebecca E, Griffin, Darren K., Kretschmer, Rafael (2022) Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species. Animals, 12 (21). Article Number 3052. ISSN 2076-2615. (doi:10.3390/ani12213052) (KAR id:99074)


Microchromosomes, once considered unimportant elements of the genome, represent fundamental building blocks of bird karyotypes. Shorebirds (Charadriiformes) comprise a wide variety of approximately 390 species and are considered a valuable model group for biological studies. Despite this variety, cytogenetic analysis is still very scarce in this bird order. Thus, the aim of this study was to provide insight into the Charadriiformes karyotype, with emphasis on microchromosome evolution in three species of shorebirds—Calidris canutus, Jacana jacana, and Vanellus chilensis—combining classical and molecular approaches. Cross-species FISH mapping applied two BAC probes for each microchromosome, GGA10–28 (except GGA16). The experiments revealed different patterns of microchromosome organization in the species investigated. Hence, while in C. canutus, we found two microchromosomes involved in chromosome fusions, they were present as single pairs in V. chilensis. We also described a new chromosome number for C. canutus (2n = 92). Hence, this study contributed to the understanding of genome organization and evolution of three shorebird species.

Item Type: Article
DOI/Identification number: 10.3390/ani12213052
Additional information: For the purpose of open access, the author(s) has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising.
Uncontrolled keywords: Bird, FISH, Comparative genomics, Microchromosome, BAC, molecular cytogenetics, Avian Karyotype
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (
Biotechnology and Biological Sciences Research Council (
SWORD Depositor: JISC Publications Router
Depositing User: JISC Publications Router
Date Deposited: 20 Jan 2023 16:22 UTC
Last Modified: 20 Nov 2023 15:42 UTC
Resource URI: (The current URI for this page, for reference purposes)

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